column_name,column_description
study,studies are: a2021 = Absinta et al. 2021; j2019 = Jaekel et al. 2019; s2019 = Schirmer et al. 2019; roche = new validation samples for this study
sample_id,sample_id
cell_id,"id for cell, corresponding to the column names of the counts matrix (this is sample_id plus droplet barcode)"
barcode,droplet barcode
sum,library size for this barcode
detected,number of genes observed for this barcode
subsets_mito_sum,total of mitochondrial genes for this barcode
subsets_mito_detected,number of mitochondrial genes observed for this barcode
subsets_mito_percent,percentage of mitochondrial genes for this barcode
total,library size for this barcode
total_spliced,total number of spliced reads for this barcode
total_unspliced,total number of unspliced reads for this barcode
qlogis_splice,logistic function of proportion of spliced genes for this barcode (in R: qlogis(pct_splice/100))
cluster,"Harmony cluster used to improve broad cell type annotation, by assigning all cells in one cluster to the majority predicted celltype"
type_broad,broad cell type annotation
sex,male or female (checked against expression of known X- and Y-linked genes)
age_at_death,"age at death, in years"
pmi_minutes,"post-mortem interval, in minutes"
donor_id,anonymised ID for donor
diagnosis,"MS diagnosis for this donor (CTR = non MS, SPMS = secondary progressive MS, PPMS = primary progressive MS, RRMS = relapsing remitting MS)"
lesion_type,"lesion type for this sample (WM = ctrl WM, NAWM = normal appearing WM, AL = active lesion, CAL = chronic lesion, CIL = chronic inactive lesion, RL = remyelinating lesion, GM = ctrl GM, NAGM = normal appearing GM, GML = cortical lesion)"