Analyses of ergot alkaloid production in Aspergillus leporis and an easD knockout and sequences for phylogenetic analyses of rugulovasine-associated genes

Data supporting Davis KA, Jones AM, and Panaccione DG. Two satellite gene clusters enhance ergot alkaloid biosynthesis capacity of Aspergillus leporis submitted to Applied and Environmental Microbiology submitted for review May 2023 

All relevant chemical data are contained in one Excel file with two tabs. The tab labeled ‘time course room temp’ contains raw quantities of three ergot alkaloids measured during the time course assay of wild-type Aspergillus leporis and supports Supplementary Figure 2. The data are listed in four columns with the first being the number of days (after inoculation of the medium) at which the samples were harvested. The second through fourth columns list the quantities (in nmol per g fungus) of lysergic acid alpha-hydroxyethylamide (LAH), chanoclavine-I, and fumigaclavine A detected in the combined solid (combination of hyphae, conidiophores, and conidia) and liquid (culture fluids) phases of samples. The tab labeled ‘easD knockout 30 C’ contains data from which Table 1 is derived. The first two columns list the quantities (in nmol per g fungus) of chanoclavine-I and fumigaclavine A detected in cultures. The third column lists the percent conversion of chanoclavine-I to fumigaclavine A calculated from the first two columns. The final column lists the strain from which the sample was measured. The data in each Excel tab are arrayed in a format ready to be copied and pasted into a JMP worksheet. 

Amino acid sequence data used for generation of the phylogenetic trees in Figure 6 and Figure 7 are listed in one Word file. The Word file contains EasQ and EasH amino acid sequences used to generate the trees in figures 6 and 7. Data for individual trees are separated by page breaks and labeled by enzyme. Data are ready to be copied and pasted for alignment and phylogenetic analyses.