Orbitrap Exploris 480 Method Summary
  

  
Global Settings
	Method Duration (min)= 11
	Use Ion Source Settings from Tune = Not Checked
	Use Static Source Gasses
	Collision Gas Type = -1
	Collision Gas pressure (mTorr)= 1
	Ion Source Type = H-ESI
	Spray Voltage = Static
	Spray Voltage: Positive Ion (V) = 3500
	Spray Voltage: Negative Ion (V) = 2500
	Sheath Gas (Arb) = 60
	Gas Mode = Static
	Infusion Mode (LC)= False
	Aux Gas (Arb) = 15
	Sweep Gas (Arb) = 2
	Ion Transfer Tube Temp (°C) = 350
	Vaporizer Temp (°C) = 400
	APPI Lamp = Not in use
	FAIMS Mode = Not Installed
	Application Mode = Small Molecule
	Pressure Mode = Standard
	Expected Peak Width (s) = 4
	Default Charge State = 1
	Advanced Peak Determination = True
	Xcalibur AcquireX enabled for method modifications = True
	Mild Trapping = False
Experiment 1
	Start Time (min) = 0
	Experiment Name = MS
	End Time (min) = 11
		


		Scan MasterScan
			Orbitrap Resolution = 15000
			Scan Range (m/z) = 100-1000
			Microscans = 1
			RF Lens(%) = 30
			Maximum Injection Time Mode = Auto
			AGC Target = Standard
			EASY-IC = Off
			DataType = Profile
			Polarity = Positive
			Source Fragmentation = False
			Scan Description = 

		Filter IntensityThreshold
			Intensity Filter Type = IntensityThreshold
			Minimum Intensity = 5000

		Filter DynamicExclusion
			Share Dynamic exclusion list with other selected dynamic exclusion filters = False
			Dynamic Exclusion Mode = Custom
			Exclude after n times = 1
			Exclusion duration (s) = 2.5
			Excl. Mass Width = ppm
			Mass tolerance low = 5
			Mass tolerance high = 5
			Exclude isotopes = True
			Perform dependent scan on single charge state per precursor only = False

		Filter TargetedInclusionMassList
			Add Mass List Targets Determined by Xcalibur AcquireX = False
			Mass list type = m/z
			Time Mode = Start/End Time
			Include Intensity Threshold = True
			Mass tolerance = ppm
			Mass tolerance Low = 5
			Mass tolerance High = 5
			Ignore charge state requirement for unassigned ions = True
			Set Activation parameters per compound = False
			Perform dependent scan on most intense ion if no targets are found = True
>>>>>>>>>>>>> Mass List Table <<<<<<<<<<<<<<
   CompoundName|            m/z|  t start (min)|   t stop (min)|      Threshold|
       Caffeine|       195.0877|              0|              5|              0|
>>>>>>>>>>>>> End Mass List Table <<<<<<<<<<<<<<

		Filter TargetedExclusionMassList
			Filter Label = Xcalibur AcquireX
			Add Mass List Targets Determined by Xcalibur AcquireX = True
			Mass list type = m/z
			Include Intensity Threshold = True
			Time Mode = Start/End Time
			Mass tolerance = ppm
			Mass tolerance Low = 5
			Mass tolerance High = 5
			Ignore charge state requirement for unassigned ions = True
			Set Activation parameters per compound = False
			Perform dependent scan on most intense ion if no targets are found = False
>>>>>>>>>>>>> Mass List Table <<<<<<<<<<<<<<
   CompoundName|            m/z|  t start (min)|   t stop (min)|      Threshold|
>>>>>>>>>>>>> End Mass List Table <<<<<<<<<<<<<<

		Data Dependent Properties
			Data Dependent Mode= Number of Scans
			Number of Dependent Scans= 5
Scan Event 1
		


		Scan ddMSnScan
			Multiplex Ions Enabled = False
			Isolation Offset = Off
			Isolation Window = Custom
			Isolation Window (m/z) = 1.5
			Maximum number of multiplexed ions = 0
			Reported Mass = Offset Mass
			Collision Energy Type = Normalized
			HCD Collision Energies (%) = 30,50,70
			Orbitrap Resolution = 15000
			Time (ms) = 54
			Microscans = 1
			RF Lens(%) = 50
			AGC Target = Standard
			Maximum Injection Time Mode = Custom
			EASY-IC = Off
			DataType = Profile
			Source Fragmentation = False
			Scan Description =