Published March 29, 2023 | Version v2
Software Open

Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis - software

  • 1. Laboratory of Biology and Information Science, School of Life Sciences, East China Normal University, Shanghai 200062, China; Laboratory for Artificial Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Polo Scientifico e Tecnologico Fabio Ferrari, Polo B, Via Sommarive 9, Povo, 38123, Trentino Alto-Adige, Italy
  • 2. Laboratory for Artificial Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Polo Scientifico e Tecnologico Fabio Ferrari, Polo B, Via Sommarive 9, Povo, 38123, Trentino Alto-Adige, Italy
  • 3. Department of Physics, Graduate School of Science, Tohoku University, 6-3 Aramaki, Aoba, Sendai 980-8578, Japan
  • 4. Laboratory of Biology and Information Science, School of Life Sciences, East China Normal University, Shanghai 200062, China;

Description

Scripts developed for the analysis of vesicles fusion experiments by high-throughput image analysis for "Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis".

-  Quick User Guide for GUV fusion Analysis.pdf is a short manual detailing the main steps to complete the analisys of imaging data by our system

- GUVfusion_Analysis.ijm is the ImageJ macro used to segment GUVs

- GUVfusion_AnalysisTemplate.R is the R template script for data elaboration and visualization. It makes use of the other two R scripts included

- GUVfusion_dataElabFunctions.R contains data elaboration functions to import raw csv and tiff data in R

-GUVfusion_plotFunctions.R contains data visualization functions.

Files

Files (1.8 MB)

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md5:430fa3d503fb70227305649462a53fb7
18.1 kB Download
md5:ef026d878495ca287347928d531affca
35.4 kB Download
md5:e2468c11fb6b6256975b4da1eb7db5ef
31.6 kB Download
md5:a7ed701a3e8aa0982715eaa9121f7581
47.3 kB Download
md5:bc923067a3fa6e87eb8f03a3284f6f4d
1.7 MB Download

Additional details

Funding

ACDC – Artificial Cells with Distributed Cores to Decipher Protein Function 824060
European Commission