Script and data for: 

Alruiz JM,  Peralta-Maraver I, Bozinovic F, Santos M, Rezende EL. (2022), Temperature adaptation and its impact on the shape of performance curves in Drosophila populations - Proc. Royal Soc. B .

Contains 5 files:

(1) code.r - R script to replicates analyses and the figures in the paper and Supplementary Material:

	1.1. Figure 1: Map with studied populations of Drosophila simulans in Chile, and graphical representation of the thermal hypotheses.

	1.2. PCA analyses and Figure 2: Principal component analyses (PCA) including Q10, maximum performance, thermal optimum, thermal breadth and critical thermal maxima, and the relationship between principal component 1 from each PCA with the monthly averaged maximum temperature and the monthly averaged thermal range. 

        1.3. Figure 3: Fitted TPCs for metabolic rate and egg-to-adult viability, and the relationship between thermal optimum and maximum performance and between thermal breadth and maximum performance for both traits. 

        1.4. Figure 4: Coadaptation between metabolism and egg-to-adult viability.

	1.5. Code to reproduce supplementary tables and supplementary figures.

(2) Datasets (4 in total)

2.1. Dataset all_TPC.xlsx: Comprises thermal performance curve descriptors for metabolic rate and egg-to-adult viability after applying mechanistic model from Rezende and Bozinovic (2019). Dataset also includes ambient temperature metrics and color code for plotting purposes.

	Columns: q10 - temperature coefficient Q10.
		  	  cte - Coeficiente of thermal expansion		                 thr - threshold temperature (Celsius degree)
	                 decay - decay value
			  ctmax - maximum critical temperature (Celsius degree)
			  max.pf - maximum performance 
			  ta.opt - optimum temperature (Celsius degree)
			  breadth.50 - thermal breadth at 50% survival (Celsius degree)
                          breadth.80 - thermal breadth at 80% survival (Celsius degree)
			  r.square - coefficient of determination from TPCs model fitting
			  n.samples - number of samples
			  pop - sampled populations
			  col - color code for plotting purposes
			  tmean - monthly averaged mean temperature (Celsius degree)
			  tmin - monthly averaged minimum temperature (Celsius degree)
			  tmax - monthly averaged maximum temperature (Celsius degree)
                          tvar - thermal variance (tmax-tmin)
			  Lat - latitude of the sampled population
			  Level - traits (vco: metabolic rate; viab: viability)

2.2. Dataset metabolism.xlsx: Raw data data of metabolic rates measured experimentally.

          Columns: pop - sampled population
			     tt - temperature trial (Celsius degree)
                           sex - sex of individuals (m = males, f= females). 
			     mass - body mass of individual in mg
			     VCO2 - mean value of measured metabolic rate in uL CO2/h
			     stdCO2 - standard deviation value of measured metabolic rate in uL CO2/h

2.3. Dataset viability.xlsx: Raw data data of egg-to-adult viability measured experimentally.

          Columns: pop - sampled population
			     ttrial- temperature trial (Celsius degree)
			     vial - vial number
			     pdate/edate - initial and final sampled dates
			     days - duration of trial in days
                           ne - number of eclosions

2.4. Dataset Temp_2018_2020.csv: Database with time series of temperature for each of the 8 sampling locations at a one-hour resolution for the period between 2018 and 2020

(3) Analyses_thermal_functions.r: r code with functions to fit TPCs using mechanistic models from Rezende and Bozinovic (2019).


References:

1. Rezende EL, Bozinovic F. (2019). Thermal performance across levels of biological organization. Philosophical Transactions of the Royal Society B, 374, 20180549.