Monitoring of Algal Bloom in Freshwater Lakes, Extraction and Evaluation of Antibacterial Compounds
- 1. Telangana Social Welfare Residential Degree College (W), Mancherial, Telangana
Description
Abstract
Cyanobacteria (Blue-green algae) is a photosynthetic bacteria found in both freshwater and marine habitats. Some of the earliest known life forms on Earth are cyanobacteria, which are also thought to be the first species to produce oxygen as a consequence of photosynthesis. They are significant primary producers in aquatic habitats, giving other creatures energy and nutrients. In industrial and agricultural activities including wastewater treatment, bioremediation, and the creation of biofuels, they are also employed. Algal blooms were tracked as part of the Open Filed Collective Project, and each month their diversity is recorded. The freshwater lake near Adilabad and the Mancherial district is being monitored as part of this study by taking water samples, which are then analyzed for physiological features like pH, daytime temperature, the presence of algae in the water, and odor. A spectrophotometer is used to determine the number of algae in the water, and a foldscope and compound microscope are used to view the cells. In order to develop the algae species in tap water, growth promoters like CaCO3 and NaNO3 are added. Following filtering, the algal mat is dried by air. The algal dry mat is pulverized and extracted using a liquid-solid mixture of methanol and ether (7:3). Chromatography is then used to examine the chemicals recovered from the extract using the solvent mixture. The Agar well diffusion method is then used to test for antibacterial activity by the crude extract. By evaluating the Zone of Inhibition against E. coli, S. typhi, and M. luteus, antibacterial activity is measured and findings are documented. The zone of inhibition is measured to be 7mm on average. The suggested framework will assist in keeping track of a water body's biodiversity of species as well as its amount of algal bloom. This will allow for the aquatic ecosystem's health to be monitored, maybe allowing for the discovery of new microbial species.
Key words - Algal Bloom, freshwater lakes, Foldscope, Spectrophotometer, Extraction, Antibacterial activity.
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- CA, CONJUGATES N. Gunaswetha Kuraganti, Sujatha Edla and Thrimothi Dasari Department of Microbiology, Kakatiya University, Warangal-506009, Telangana, India.
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