Published March 26, 2024 | Version v2
Dataset Open

A multiomic characterization of the leukemia cell line REH using short- and long-read sequencing

  • 1. Department of Medical Sciences and Science for Life Laboratory, Uppsala University, Uppsala, Sweden
  • 2. National Genomics Infrastructure hosted by Science for Life Laboratory, Uppsala University, Uppsala, Sweden
  • 3. Department of Immunology, Genetics and Pathology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden

Description

This is a public repository containing secondary datasets described in the publication "A multiomic characterization of the leukemia cell line REH using short- and long-read sequencing". Primary data for this project are available at NCBI/SRA under the BioProject accession numbers PRJNA600820 and PRJNA834955, and include the following sequencing datasets:

REH cell line:

  • PacBio WGS
  • ONT Ultralong WGS
  • Illumina short-read PCR-free WGS
  • IsoSeq RNA-seq
  • Illumina short-read RNA-seq

GM12878 cell line:

  • Illumina short-read RNA-seq

This dataset includes the following files:

Depth of Coverage analysis

  • Output from `samtools coverage`: samtools.coverage.illumina.txt, samtools.coverage.ont.txt, samtools.coverage.pb.txt
  • Output from `copycat` (binned coverage): copycat.ont.coverage.10kb.csv, copycat.pb.coverage.10kb.csv, copycat.pcrfree.coverage.10kb.csv

Structural Variant (SV) callsets

  • Raw: illumina.tiddit.vcf, ont.sniffles.vcf, pb.sniffles.vcf
  • Filtered: REH.svs.filtered.csv

SNV callsets

  • Filtered and annotated: REH.mutect.filtered.ann.vcf.gz

Fusion gene callsets

  • Short-read: GM12878.fusionreport.txt, illumina.all.txt, illumina.filtered.csv, REH.arriba.fusions.tsv, REH.fusioncatcher.fusion-genes.txt, REH.pizzly.txt, REH.squid.fusions.annotated.txt, REH.starfusion.abridged.tsv, REH.pdf
  • Long-read: cupcake.long.csv, cupcake.std.csv, jaffa_results.csv
  • Filtered: REH.fusions.filtered.csv
     

 

Files

copycat.ont.coverage.10kb.csv

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