README
DATE: 27/06/2022
TITLE:  An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
AUTHORS: Yajun Wang, Michael Abrouk, Spyridon Gourdoupis, Dal-Hoe Koo, John Raupp, Miroslava Karafiátová, István Molnár, Kateřina Holušová, Jaroslav Doležel, Naveenkumar Athiyannan, Emile Cavalet-Giorsa, Łukasz Jaremko, Jesse Poland, Simon G. Krattinger
CONTACT: yajun.wang@kaust.edu.sa, simon.krattinger@kaust.edu.sa

The file 'ThatcherLr9_whole_genome_assembly.fasta.gz' contains DNA sequence of the whole genome assembly of hexaploid bread wheat (Triticum aestivum L. AABBDD, 2n = 6x = 42) cv. ThatcherLr9. The file 'Aegilops_umbellulata_TA1851_whole_genome_assembly.fasta.gz' contains DNA sequence of the whole genome assembly of diploid Aegilops umbellulata (UU, 2n = 2x = 14) accession TA1851. Data was produced using PacBio HiFi sequencing and assembled with Hifiasm.
The files 'ThatcherLr9_IsoSeq_cluster_hq.fasta.gz' and 'TA5605_IsoSeq_cluster_hq.fasta.gz' contain high-quality transcript isoforms from Puccinia triticina infected leaf samples of ThatcherLr9 and TA5605, respectively. Data was produced using PacBio long-read isoform sequencing (Iso-Seq) and processed following the IsoSeq pipeline (v.3.; https://github.com/PacificBiosciences/IsoSeq).
The Lr9 CDS and genomic sequences are present in FASTA format.


Provided files:
- ThatcherLr9_whole_genome_assembly.fasta.gz
- Aegilops_umbellulata_TA1851_whole_genome_assembly.fasta.gz
- ThatcherLr9_IsoSeq_cluster_hq.fasta.gz
- TA5605_IsoSeq_cluster_hq.fasta.gz
- TA5605-chrom2B.fasta.gz
- Lr9_CDS_genomic_sequence.fasta