Published March 3, 2023 | Version v1
Dataset Open

An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

  • 1. King Abdullah University of Science and Technology
  • 2. Kansas State University
  • 3. Centre of the Region Haná for Biotechnical and Agricultural Research

Description

The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with novel disease-resistance genes. Here, we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata. We establish that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Ae. triuncialis, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.

Notes

  • The file 'ThatcherLr9_whole_genome_assembly.fasta.gz' contains the DNA sequence of the whole genome assembly of hexaploid bread wheat (Triticum aestivum L. AABBDD, 2n = 6x = 42) cv. ThatcherLr9. The file 'Aegilops_umbellulata_TA1851_whole_genome_assembly.fasta.gz' contains the DNA sequence of the whole genome assembly of diploid Aegilops umbellulata (UU, 2n = 2x = 14) accession TA1851. Data was produced using PacBio HiFi sequencing and assembled with Hifiasm.
  • The files 'ThatcherLr9_IsoSeq_cluster_hq.fasta.gz' and 'TA5605_IsoSeq_cluster_hq.fasta.gz' contain high-quality transcript isoforms from Puccinia triticina infected leaf samples of ThatcherLr9 and TA5605, respectively. Data was produced using PacBio long-read isoform sequencing (Iso-Seq) and processed following the IsoSeq pipeline (v.3.; https://github.com/PacificBiosciences/IsoSeq).
  • The Lr9 CDS and genomic sequences are present in FASTA format.

Funding provided by: King Abdullah University of Science and Technology
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004052
Award Number: Office of Sponsored Research OSR-CRG2018-3768

Funding provided by: H2020 Marie Skłodowska-Curie Actions
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100010665
Award Number: Grant award 'AEGILWHEAT,' H2020-MSCA-IF-2016-746253

Funding provided by: European Regional Development Fund
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100008530
Award Number: Project "Plants as a tool for sustainable global development" CZ.02.1.01/0.0/0.0/16_019/0000827

Files

README.txt

Files (5.7 GB)

Name Size Download all
md5:4bbd232d7328ad8f63dcc0de3e91c244
1.1 GB Download
md5:e46fc8277b7a4f1ff7443bc189eacc2c
19.6 kB Download
md5:7eeb6546dc84f01fd1b9d678e9be26cb
1.6 kB Preview Download
md5:f20de920023ea0a449b4bd61ebc815cd
219.4 MB Download
md5:21ce70c2a01b07def8b2703d7981ac41
237.3 MB Download
md5:ed021d75f5a0dbc32018903e1d706de2
250.1 MB Download
md5:5d06d56dda29e63fb72a36d616a28c7c
3.9 GB Download