Dotblots of the secretion assays of HT29-N2 cells KO for STX2 (First assay). Control and the STX2 KO cell line express mucin5AC.GFP (clone 10).

The secreted fractions were passed trough a nitrocellulose membrane on a dot blot devise. The folder has one image of GFP emission, one image of the emission of immunobloting against mucin5-AC and one of the membrane.

Samples in the dot blot are loaded as follows:

Columns:
First: WT
Second: Test sample from a different experiment.
Third: STX2 KO clone 11
Fourth and Fifth: Test samples from a different experiment.

Rows:
Each row is an independent secretion assay.

First 3 rows are 3 basal conditions
Last 3 rows are 3 ATP-stimulated condition
