Dotblots of the secretion assays of HT29-N2 cells KO for STX2 (Second assay). Control and STX2 KO cell lines express mucin5AC.GFP (clone 10).

The secreted fractions were passed trough a nitrocellulose membrane on a dot blot devise. The folder has one image of GFP emission, one image of the emission of immunobloting against mucin5-AC and one of the membrane.

Samples in each dot blot are loaded as follows:

Columns:
First: WT
Second: STX2 KO clone 1
Third: STX2 KO clone 11

Rows:
Each row is an independent secretion assay.

First 3 rows are 3 basal conditions
Last 3 rows are 3 ATP-stimulated condition
