Inside this folder there are WB blots of lysates of the secretion assay of WT and STX2 KO cells. Both cell lines express mucin5AC.GFP (clone 10).

The 3 replicates were loaded in the same gel (separated by a lane with protein ladder). The name of the folder indicates the antibody used.

Membranes were cut horizontally to blot with anti stx2 and beta tubulin antibodies.

Starting from the ladder samples are as follow:

1 to 4: replicate 1.

1. WT basal secretion
2. WT ATP-stimulated
3. STX2 KO clone 11 basal secretion
4. STX2 KO clone 11 ATP-stimulated
5. ladder.

6 - 15. As 1 to 5 for replicates number 2 and 3.


