Comparative genome and proteome analysis of Labeo rohita from Halda river

The main purpose of this study to evaluate the genomic and proteomic comparison between Labeo rohita of Halda River with L. rohita and other Indian major carps from other water bodies. In this study genetic and proteomic variation of Labeo rohita determined by functional genomics and proteomics through computational biology and bioinformatics. This is the advance level of consequent study which carried over the whole genome sequencing shotgun analysis done by the students of MS. Fisheries & Limnology Session 2017-2018 previous year. In chromosome level assembly of L. rohita total number of bases of the genome found is 1,159,574,983 . No. of sequence in my study is 2401 which indicates less fragmented better genome than other reference sequence. N50 is 35.5 mbp2, L50 is smallest in my study 9, which is better because of having large sized scaffolds. Among 47,568 genes found in my study up to chromosome level, majority of those are placed in chromosome. Approximately 1010 annotated genes are protein coding and directly or indirectly responsible for muscle growth of L. rohita from Halda River. According to the refseq BAU-BD-2019 approximately 82 unique genes are found in L. rohita from Halda River. 29.27% of those genes are unplaced; 24 genes. Among 82 genes, 58 (70.73%) genes are placed in chromosome. Max. number of unique muscle genes lies in chr. 2 indicating high degree of polymorphism and mutations while no uniqueness in chr. 10, chr. 13, chr. 22 & chr. 23 indicates less polymorphism and mutations in those regions. This study carried out the divergence, unique genetic features of one of the most important common carp ‘Rohu’ from Halda River.