LOCUS       pMIH179        6787 bp DNA     circular SYN 07-FEB-2023
DEFINITION  HA-CD55-TM-TAGNTF2-mTurqIn.
ACCESSION   .
VERSION     .
KEYWORDS    HA-CD55-TM-TAG(NTF2)-mTurq(In)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 7, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     728..790
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             791..817
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    839..1024
                     /label=SCR1
     misc_feature    1025..1216
                     /label=SCR2
     misc_feature    1217..1402
                     /label=SCR3
     misc_feature    1403..1591
                     /label=SCR4
     misc_feature    1607..1609
                     /label=TAG
     CDS             1631..1777
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             1787..2500
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    2534..2758
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2890..3478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3807..3854
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4086..4874)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      4923..5061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5078..5269
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     CDS             complement(5329..6189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6190..6294)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6321..6776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg gcttggggat
      721 atccaccatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
      781 cactggtgac tatccatatg atgttccaga ttatgctggg gcccagccgg ccagatctga
      841 ctgtggcctt cccccagatg tacctaatgc ccagccagct ttggaaggcc gtacaagttt
      901 tcccgaggat actgtaataa cgtacaaatg tgaagaaagc tttgtgaaaa ttcctggcga
      961 gaaggactca gtgatctgcc ttaagggcag tcaatggtca gatattgaag agttctgcaa
     1021 tcgtagctgc gaggtgccaa caaggctaaa ttctgcatcc ctcaaacagc cttatatcac
     1081 tcagaattat tttccagtcg gtactgttgt ggaatatgag tgccgtccag gttacagaag
     1141 agaaccttct ctatcaccaa aactaacttg ccttcagaat ttaaaatggt ccacagcagt
     1201 cgaattttgt aaaaagaaat catgccctaa tccgggagaa atacgaaatg gtcagattga
     1261 tgtaccaggt ggcatattat ttggtgcaac catctccttc tcatgtaaca cagggtacaa
     1321 attatttggc tcgacttcta gtttttgtct tatttcaggc agctctgtcc agtggagtga
     1381 cccgttgcca gagtgcagag aaatttattg tccagcacca ccacaaattg acaatggaat
     1441 aattcaaggg gaacgtgacc attatggata tagacagtct gtaacgtatg catgtaataa
     1501 aggattcacc atgattggag agcactctat ttattgtact gtgaataatg atgaaggaga
     1561 gtggagtggc ccaccacctg aatgcagagg agtcgacgaa caaaaataga tctcagaaga
     1621 ggatctgaat gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc
     1681 ctttaaggtg gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct
     1741 tatcatcctc atcatgcttt ggcagaagaa gccacgtcct gcaggggtga gcaagggcga
     1801 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1861 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1921 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc
     1981 ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2041 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2101 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2161 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2221 catcagcgac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     2281 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     2341 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2401 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2461 cgccgccggg atcactctcg gcatggacga gctgtacaag gctagctagg cggccgctcg
     2521 agatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2581 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2641 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2701 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2761 tctgaggcgg aaagaaccag tggcggtaat acggttatcc acagaatcag gggataacgc
     2821 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2881 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2941 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3001 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3061 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3121 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3181 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3241 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3301 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3361 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3421 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3481 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3541 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3601 aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc
     3661 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     3721 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     3781 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg
     3841 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     3901 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     3961 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     4021 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     4081 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     4141 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     4201 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
     4261 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     4321 gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct
     4381 ggcgcgagcc cctgatgctc ttgatcatcc tgatcgacaa gaccggcttc catccgagta
     4441 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4501 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4561 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4621 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4681 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     4741 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     4801 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     4861 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     4921 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggaggcggc
     4981 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     5041 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     5101 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     5161 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5221 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     5281 tttcaataaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5341 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5401 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5461 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5521 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5581 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5641 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5701 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5761 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5821 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5881 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5941 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6001 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6061 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6301 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6361 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6421 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6481 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6541 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6601 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6661 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6721 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttacgc
     6781 gcgcgtt
//
