Gene expression simulator/predictor for Ciona early embryos (ver. 2.1)

Step 1. Specify distribution patterns of upstream regulators (hit "Normal embryo" to reproduce patterns in normal embryos)
Step 2. Specify regulatory functions of downstream target genes (hit "Normal embryo" to reproduce regulatory functions in normal embryos)
Step 3. Then hit 'Computation' to calculate gene expression patterns in 16-cell and 32-cell embryos
(Option) Illustrations for 16-cell ane 32-cell embryos and cell lineages
(Option) Try to add hypothetical regulators (Hyp. X, Hyp. Y, Hyp. Z) and hypothetical targets (Hyp. A, Hyp. B, Hyp. C) to this simulation

‹Step 1› Regulators ‹Step 3› Targets
⇐ Normal
⇐ All ON "1", expressed
⇐ All OFF "0", not expressed
Gata.a Macho-1 CA-Raf beta-catenin Sox1/2/3 Zfpm Foxa.a Foxd Foxtun2 Tbx6-r.a Tbx6-r.b Prdm1-r Tfap2-r.b Hes.a Fgf9/16/20 Efna.d Admp Gdf1/3-r Wnttun5 Hyp. X Hyp. Y Hyp. Z Cell Lhx3/4 Dickkopf Neurogenin Snail Wnt3 Wnt5 Bmp3 Hes.b Zic-r.b Dmrt.a Otx Dlx.b Nodal Hyp. A Hyp. B Hyp. C
16-A5.1
16-A5.2
16-B5.1
16-a5.3
16-a5.4
16-b5.3
16-b5.4
32-A6.1
32-A6.2
32-A6.3
32-A6.4
32-B6.1
32-B6.2
32-B6.4
32-a6.5
32-a6.6
32-a6.7
32-a6.8
32-b6.5
32-b6.6
32-b6.7
32-b6.8

‹Step 2› Regulatory Functions [Hit "Normal Embryo" or specify product terms in the boxes below (use "&" for "∩" and "ˆ" for negation)]
Lhx3/4 ∪ ∪ ∪ ∪
Dickkopf ∪ ∪ ∪ ∪
Neurogenin ∪ ∪ ∪ ∪
Snail ∪ ∪ ∪ ∪
Wnt3 ∪ ∪ ∪ ∪
Wnt5 ∪ ∪ ∪ ∪
Bmp3 ∪ ∪ ∪ ∪
Hes.b ∪ ∪ ∪ ∪
Zic-r.b ∪ ∪ ∪ ∪
Dmrt.a ∪ ∪ ∪ ∪
Otx ∪ ∪ ∪ ∪
Dlx.b ∪ ∪ ∪ ∪
Nodal ∪ ∪ ∪ ∪
Hyp. A ∪ ∪ ∪ ∪
Hyp. B ∪ ∪ ∪ ∪
Hyp. C ∪ ∪ ∪ ∪