# Title of Dataset:
Polymer nanoparticles pass the plant interface

Brief summary of dataset contents, contextualized in experimental procedures and results.

we prepared well-defined block copolymer nanoparticles by aqueous dispersion polymerisation. A fluorophore was incorporated via hydrazone formation and uptake into roots and protoplasts of Arabidopsis thaliana was investigated using confocal microscopy. Here we show that uptake is inversely proportional to nanoparticle size. Positively charged particles accumulate around root surfaces and are not taken up by roots or protoplasts, whereas negatively charged nanoparticles accumulate slowly and become prominent over time in the xylem of intact roots. Neutral nanoparticles penetrate rapidly into intact cells at the surfaces of plant roots and into protoplasts, but xylem loading is lower than for negative nanoparticles. These behaviours differ from those of animal cells and our results show that despite the protection of rigid cell walls, plants are accessible to nanoplastics in soil and water.


## Description of the Data and file structure

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Describe relationship between data files, missing data codes, other abbreviations used. Be as descriptive as possible.

We present here only the summary raw data from our quantitative PCR experiments and the raw image files from our confocal microscopy. The associated README method files explain the full methodology in each case.  There is also a script to explain the filenames.


## Sharing/access Information
A full preprint of the manuscript is available at BioRxiv https://doi.org/10.1101/2022.03.24.485656