This readme.txt file was generated on 2022-09-22 by K. Skogen and E. Lewis
 
GENERAL INFORMATION
 
1. Title of Dataset: Oenothera Section Calylophus population genetic study
 
2. Author Information
            A. Principal Investigator Contact Information
                        Name: Krissa Skogen
                        Institution: Clemson University
                        Address: 132 Long Hall Clemson, SC 29631 USA
                        Email: kskogen@clemson.edu
 
            B. Associate or Co-investigator Contact Information
                        Name: Emily Lewis
                        Institution: Washington State University
                        Address: 103 Neill Hall Pullman, WA 99163-3113 USA
                        Email: emily.m.lewis@wsu.edu
 
            C. Associate or Co-investigator Contact Information
                        Name: Jeremie Fant
                        Institution: Chicago Botanic Garden
                        Address: 1000 Lake Cook Road Glencoe, IL, 60022 USA
                        Email: jfant@chicagobotanic.org
 
            D. Associate or Co-investigator Contact Information
                        Name: Michael Moore
                        Institution: Oberlin College
                        Address: Science Center K111 Oberlin, OH 44074 USA
                        Email: mmoore@oberlin.edu
 
3. Date of data collection (single date, range, approximate date) 2014 - 2015
 
4. Geographic location of data collection: New Mexico and Texas, USA 
 
5. Information about funding sources that supported the collection of the data: NSF DEB 1342873
 
 
SHARING/ACCESS INFORMATION
 
1. Licenses/restrictions placed on the data: None
 
2. Links to publications that cite or use the data: Citation provided upon acceptance for publication
 
3. Links to other publicly accessible locations of the data: None
 
4. Links/relationships to ancillary data sets: None
 
5. Was data derived from another source? No 
 
6. Recommended citation for this dataset: Citation provided upon acceptance for publication
 
 
DATA & FILE OVERVIEW
 
1. File List:
 
-FloralMorphGC_noYA_2021.csv: Floral morphology measurements for 333 growth-chamber grown O. hartwegii subsp. filifolia and O. gayleana plants, including corolla diameter, floral flare, etc.
 
-FloralMorphGC_noYA_2022.csv: Floral morphology measurements for 333 growth-chamber grown O. hartwegii subsp. filifolia and O. gayleana plants, including corolla diameter, floral flare, etc., with herkogamy left as blank if there was no style length measurement recorded.
 
-FST_GeogDistance.csv: For each pair of study sites, geographic distance (km), calculated FST, and linearized FST. 
 
-GC_Crosses_December_2021.csv: The total seeds produced by each cross performed, with information on maternal population and cross type. 
 
-GCWild_FloralMorphologyMaster.csv: Floral morphology measurements for 404 wild plants taken at in wild populations and 199 growth chamber plants grown from seeds collected at these wild populations. 
 
-GeneticAnalysis_Chloro.csv: Population genetic parameters for the chloroplast haplotype analysis.
 
-GeneticAnalysis.csv: Population genetic parameters for the nuclear microsatellite genotype analysis.
 
-Matrix_Distance.csv: Matrix of pairwise geographic distances (km) between each of the populations. 
 
-Matrix_FSTFili_Lin.csv: Matrix of pairwise linearized FST values between each of the O. hartwegii subsp. filifolia populations. 
 
-Matrix_FstGay_Lin.csv: Matrix of pairwise linearized FST values between each of the O. gayleana populations.
 
2. Relationship between files, if important: 
 
3. Additional related data collected that was not included in the current data package: None
 
4. Are there multiple versions of the dataset? FloralMorphGC_noYA_2021 and FloralMorphGC_noYA_2022 are very similar datasets, with only one column having different values. For ease of statistical computing, the 2022 version was saved as a new document. 
 
 
METHODOLOGICAL INFORMATION
 
1. Description of methods used for collection/generation of data: Details of data collection are in the Methods section of the manuscript (citation provided upon acceptance for publication)
 
2. Methods for processing the data: Raw data files were processed in R scripts provided
 
3. Instrument- or software-specific information needed to interpret the data: NA
 
4. Standards and calibration information, if appropriate: NA
 
5. Environmental/experimental conditions: See details provided in (citation provided upon acceptance for publication)
 
6. Describe any quality-assurance procedures performed on the data: all data were entered and verified to correct data entry errors
 
7. People involved with sample collection, processing, analysis and/or submission: E. M. Lewis, K. A. Skogen, J. Fant, and M. Moore.
 
 
DATA-SPECIFIC INFORMATION FOR: FloralMorphGC_noYA_2021.csv:
1. Number of variables: 20
2. Number of cases/rows: 334
3. Variable List: 
Species: G = O. gayleana, F = O. hartwegii subsp. filifolia
Population & Site: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D
Individual: individual plant ID number
MatLine: Maternal line ID number
Flower: flower measured, 1 = first, 2 = second, 3 = third, 4 - fourth 
Date: Date data were collected
corolla_diameter_1: corolla diameter (mm) measurement perpendicular to corolla_diameter_2 (mm)
corolla_diameter_2: corolla diameter (mm) measurement perpendicular to corolla_diameter_1
Floral_flare: diameter (mm) of the opening of the floral tube
Filament_length1: length of first filament (mm)
Filament_length2: length of second filament (mm)
Style_length: length from floral flare to end of style (mm)
Tube_length: length from floral flare to insertion at ovary (mm)
H: Simplified negative, neutral, or positive herkogamy 
Herkogamy: the physical separation between anthers and the stigma, was measured as the distance between the tallest anther and the stigma (mm)
Notes: Notes taken during data collection
Location: Location of plants included, GC = growth chamber
PCA_Herk: Notation for R computations, yes = has style and filament measurement and can be used for herkogamy; no = missing one or more measurements.
 
DATA-SPECIFIC INFORMATION FOR: FloralMorphGC_noYA_2022.csv:
1. Number of variables: 20
2. Number of cases/rows: 334
3. Variable List: 
Species: G = O. gayleana, F = O. hartwegii subsp. filifolia
Population: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Individual: individual plant ID number
MatLine: Maternal line ID number
Flower: flower measured, 1 = first, 2 = second, 3 = third, 4 - fourth 
Date: Date data were collected
corolla_diameter_1: corolla diameter (mm) measurement perpendicular to corolla_diameter_2 (mm)
corolla_diameter_2: corolla diameter (mm) measurement perpendicular to corolla_diameter_1
Floral_flare: diameter (mm) of the opening of the floral tube
Filament_length1: length of first filament (mm)
Filament_length2: length of second filament (mm)
Style_length: length from floral flare to end of style (mm)
Tube_length: length from floral flare to insertion at ovary (mm)
H: Simplified negative, neutral, or positive herkogamy 
Herkogamy: the physical separation between anthers and the stigma, was measured as the distance between the tallest anther and the stigma (mm)
Notes: Notes taken during data collection
Location: Location of plants included, GC = growth chamber
PCA_Herk: Notation for R computations, yes = has style and filament measurement and can be used for herkogamy; no = missing one or more measurements.
 
DATA-SPECIFIC INFORMATION FOR: FST_GeogDistance.csv:
1. Number of variables: 6
2. Number of cases/rows: 57
3. Variable List: 
Pop_pair: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Distance: distance between populations (km)
Species: G = O. gayleana, F = O. hartwegii subsp. filifolia
FST: FST as calculated by GenAlEx
FST_Lin_Spag: FST linearized by Spaghedi program
FST_Lin: FST linearized normally
 
DATA-SPECIFIC INFORMATION FOR: GC_Crosses_December_2021.csv:
1. Number of variables: 11
2. Number of cases/rows: 873
3. Variable List: 
CrossID: A unique number given to each cross
Species: G = O. gayleana, F = O. hartwegii subsp. filifolia
MaternalPop: Wild population the plant was descended from
MaternalLine: Maternal line the plant was descended from (G = species, YA = maternal population, 3 = third maternal line from G_YA, 1 = first plant from G_YA_3, for G_YA_3_1)
Maternal: Maternal plant (pollen recipient)
Paternal: Paternal plant (pollen donor)
PaternalPop: Paternal 
Cross: Type of cross (GEIT = geitonomous, SIB = Sibling, BW = Between populations, WIN = Within the same population, AUT = autogamous)
TotSeeds: Total seeds produced from cross
Date: Date cross was conducted
 
DATA-SPECIFIC INFORMATION FOR: GCWild_FloralMorphologyMaster.csv:
1. Number of variables: 18
2. Number of cases/rows: 604
3. Variable List: 
Species: G = O. gayleana, F = O. hartwegii subsp. filifolia
Population: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Site: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Individual: individual plant ID number
MatLine: Maternal line ID number
Flower: flower measured, 1 = first, 2 = second, 3 = third, 4 - fourth 
Date: Date data were collected
corolla_diameter_1: corolla diameter (mm) measurement perpendicular to corolla_diameter_2 (mm)
corolla_diameter_2: corolla diameter (mm) measurement perpendicular to corolla_diameter_1
Floral_flare: diameter (mm) of the opening of the floral tube
Filament_length1: length of first filament (mm)
Filament_length2: length of second filament (mm)
Style_length: length from floral flare to end of style (mm)
Tube_length: length from floral flare to insertion at ovary (mm)
H: Simplified negative, neutral, or positive herkogamy 
Notes: Notes taken during data collection
Location: Location of plants included, GC = growth chamber, Wild = wild population sampled at study site
 
DATA-SPECIFIC INFORMATION FOR: GeneticAnalysis_Chloro.csv:
1. Number of variables: 10
2. Number of cases/rows: 19
3. Variable List: 
Sp: Species, F = O. hartwegii subsp. filifolia, G = O. gayleana
Pop: Population, CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Scale: Landscape or local scale (Yeso hills)
N: Number of individuals
Na: Number of alleles
Ne: Number of effective alleles
I: Information index
Hallo_Total: total number of haplotypes identified
Unique: Number of haplotypes found only in that population
 
DATA-SPECIFIC INFORMATION FOR: GeneticAnalysis.csv:
1. Number of variables: 15
2. Number of cases/rows: 19
3. Variable List: 
Sp: Species, Ohf = O. hartwegii subsp. filifolia, G = O. gayleana
Pop: Population, CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Scale: Landscape or local scale (Yeso hills)
N = Number of individuals
Na = Number of alleles
Ne = Number of effective alleles
I = Information index
Ho = Observed heterozygosity
He = Expected heterozygosity
uHe = Unbiased expected heterozygosity
Fis = Fixation index
Prv = number of private alleles found in that population
Avg = Average
Relatedness
 
DATA-SPECIFIC INFORMATION FOR: Matrix_Distance.csv:
1. Number of variables: 8
2. Number of cases/rows: 9 
3. Variable List: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Pairwise genetic distance given in km
 
DATA-SPECIFIC INFORMATION FOR: Matrix_FSTFili_Lin.csv:
1. Number of variables: 8
2. Number of cases/rows: 9 
3. Variable List: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Pairwise FST values given
 
DATA-SPECIFIC INFORMATION FOR: Matrix_FstGay_Lin.csv:
1. Number of variables: 8
2. Number of cases/rows: 9 
3. Variable List: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw
Pairwise FST values given