This readme file was generated on 2022-08-12 by Tabea Hildebrand


GENERAL INFORMATION

Title of Dataset: "Data for: Transformation of dissolved organic matter by two Indo-Pacific sponges"


Corresponding Author Information
Name: Tabea Hildebrand
ORCID: 0000-0002-1317-1656
Institution: Helmholtz Institute for Functional Marine Biodiversity (HIFMB)
Email: tabea.hildebrand@uol.de


Date of data collection: July and August 2019

Geographic location of data collection: Gab Gab in Apra Harbour, Guam, USA (13°26'45.0"N 144°38'28.0"E)

Information about funding sources that supported the collection of the data: HIFMB, a collaboration between the Alfred-Wegener-Institute, Helmholtz-Center for Polar and Marine Research, and the Carl-von-Ossietzky University Oldenburg, initially funded by the Ministry for Science and Culture of Lower Saxony and the Volkswagen Foundation through the “Niedersächsisches Vorab” grant program (grant number ZN3285)




SHARING/ACCESS INFORMATION

Links to publications that cite or use the data: 

Recommended citation for this dataset: Hildebrand, Tabea et al. (2022), Data for: Transformation of dissolved organic matter by two Indo-Pacific sponges, Dryad, Dataset, https://doi.org/10.5061/dryad.m0cfxpp6v



DATA & FILE OVERVIEW

Short File List (more specific information below): 
- Bacterioplankton_counts.csv: cellcounts of bacterioplankton, 
- Bulk_measurements.csv: values of bulk measurements, like dissolved organic carbon, total dissolved nitrogen etc.
- Metadata_Guam.csv: Metadata table describing the samples
- relative_peak_intensities_of_MF_per_sample.txt: matrix of the relative peak intensities from every observed molecular formula  per sample



Relationship between files: Metadata_Guam.csv gives information on the samples for Bulk_measurements.csv and relative_peak_intensities_of_MF_per_sample.txt




METHODOLOGICAL INFORMATION

Please read Material and Methods in cited publication with title "Transformation of dissolved organic matter by two Indo-Pacific sponges" (Hildebrand et al. 2022, Limnol. Oceanogr.)




DATA-SPECIFIC INFORMATION FOR: Bacterioplankton_counts.csv

Number of columns: 8

Number of rows: 26

Variable List:
	SampleName - name of the sample
	Cellcount - number of counted bacterioplankton
	Treatment - consists out of sponge species and sample type
	Sponge - RG = Rhabdastrella globostellata, MS = Melophlus sarasinorum, BOTH = off reef
	IN_EX_OFF - Sample type: IN = inhalant, EX = exhalant, OFF = off reef
	Replicate - A-E, we have sampled 5 different specimen per sponge species and sample type, but they are not necessarily equivialent to the replicates numbered in the meta data table and the rest of the data, which is why we gave the replicates letters instead of numbers
	SamplingDate - End of August 2019
	Depth - 1 to 2 meters for R. globostellata, 5 to 10 m for M. sarasinorum, 2 m for off reef water

Specialized formats or other abbreviations used: 
	RG = Rhabdastrella globostellata, MS = Melophlus sarasinorum, BOTH = off reef
	IN = inhalant, EX = exhalant, OFF = off reef



DATA-SPECIFIC INFORMATION FOR: Bulk_measurements.csv

Number of columns: 9

Number of rows: 41

Variable List: 
	SampleName - name of sample (see Metadata_Guam.csv for more information)
	DOC_MEAN - average of two technical replicates from DOC measurements in umol/l
	TN_MEAN - average of two technical replicates from TN measurements in umol/l
	ExEff_DOC - Extraction efficiency of DOC
	ExEff_TN - Extraction efficiency of TN
	NOx - NOx in umol/l
	DON - DOC = TN - NOx (umol/l)
	14Cage - radiocarbon age (years), NA show samples, which were not measured

Specialized formats or other abbreviations used: DOC = dissolved organic carbon, TN = total dissolved nitrogen, DON = dissolved organic nitrogen



DATA-SPECIFIC INFORMATION FOR: Metadata_Guam.csv

Number of columns: 8

Number of cases/rows: 41

Variable List:
	SampleName - name of the sample
	SpongeSpecies - species of sponge: RG = Rhabdastrella globostellata, MS = Melophlus sarasinorum
	IN_EX_OFF - Sample type: IN = inhalant, EX = exhalant, OFF = off reef
	Replicate - number of replicate; for the sponges it is based on specimen, meaning that for example RG_IN_1 fits to RG_EX_1
	depth - depth (m) of sample taken
	samplingday - day of when sample was taken (DD/MM/YYYY)
	volume_cm3 - volume of the sponge specimen
	weather - weather conditions of the sampling time

Specialized formats or other abbreviations used: 
	RG = Rhabdastrella globostellata, MS = Melophlus sarasinorum
	IN = inhalant, EX = exhalant, OFF = off reef



DATA-SPECIFIC INFORMATION FOR: relative_peak_intensities_of_MF_per_sample.txt

Number of variables: 3041

Number of cases/rows: 41

Variable List: 
	SampleName - name of the sample (see Metadata_Guam.csv for more information)
	column B until column DLY - molecular formulas obtained from FT-ICR-MS measurements and the use of the tool ICBM-OCEAN (Merder et al. 2020)
	they show the relative peak intensities of every molecular formula per sample

Specialized formats or other abbreviations used: 
	RG = Rhabdastrella globostellata, MS = Melophlus sarasinorum
	IN = inhalant, EX = exhalant, OFF = off reef