D3.2 Toxicological data matrix

A targeted literature study was performed to assess the toxicological evidence for a selection of cardiotoxic chemicals, for which the results were collected in a data matrix. The objective of this document is to describe the methodology of the literature study, as well as to provide a summary and description of the main results collected in the data matrix. Chemicals for the literature study were selected from an earlier review. These chemicals elicited heart failure related effects in vitro, such as effects on cardiomyocyte viability, contractility, hypertrophy, and myopathy. The data matrix contains toxicological potency estimates for these relevant in vitro endpoints, as well as study results of in vivo toxicity assays. Relevant study characteristics were collected, such as species, cell type, dosing range, incubation time, and main outcome, facilitating comparison between chemicals and therefore prioritization efforts. The data matrix can therefore help the project in various stages, ranging from chemical selection to validation, while providing a framework with mechanistic data that can explain and integrate cardiotoxicity pathways with heart failure outcomes seen in exposed populations.