This readme file was generated on 2022-09-09 by Virginia Marugan-Hernandez GENERAL INFORMATION 1. Title of Dataset: Data from: Reduction of chickens use to perform in vitro pre-screening of novel anticoccidial compounds by miniaturisation and increased throughput of the current Eimeria tenella model 2. Author Information Corresponding Investigator Name: Virginia Marugan-Hernandez Institution: Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom Email: vhernandez@rvc.ac.uk Co-investigator Name: Sara Arias-Maroto Institution: Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom Co-investigator Name: Kelsilandia Aguiar-Martins Institution: Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom Co-investigator Name: Javier Regidor-Cerrillo Institution: SALUVET-Innova S.L., Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain. Co-investigator Name: Luis Ortega-Mora Institution: SALUVET-Innova S.L., Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain. 3. Date of data collection: 01/2022-06/2022 4. Geographic location of data collection: Brookmans Park, Hatfield AL9 7TA, UK 5. Information about funding sources that supported the collection of the data: NC3Rs Skills and Knowledge Transfer grant 6. Recommended citation for this dataset: Arias-Maroto, Sara et al. (2022), Data from: Reduction of chickens use to perform in vitro pre-screening of novel anticoccidial compounds by miniaturisation and increased throughput of the current Eimeria tenella model, Dryad, Dataset DATA & FILE OVERVIEW 1. Description of dataset This dataset contains all the files with the raw data of the quantitative PCRs used to analyse Eimeria tenella growth in cell culture. 2. File List: File 1 Name: 1st_EXPERIMENT_-_2hpi_DMSO_-_ROB_20.pcrd File 1 Description: raw qPCR data - first biological replicate (experiment 1)- 2hpi DMEM, DMSO, AMP 50, AMP 20, AMP 5, AMP 1, ROB 50, ROB 20. File 2 Name: 1st_EXPERIMENT_-_2hpi_ROB_5_-_24hpi_AMP_20.pcrd File 2 Description: raw qPCR data - first biological replicate (experiment 1)- 2hpi ROB 5, ROB 1, SAL 50, SAL 20, SAL 5, SAL 1 and 24hpi AMP 50, AMP 20 File 3 Name: 1st_EXPERIMENT_-_24hpi_AMP_5_-_SAL_20.pcrd File 3 Description: raw qPCR data - first biological replicate (experiment 1)- 24hpi AMP 5, AMP 1, ROB 50, ROB 20, ROB 5, ROB 1, SAL 50, SAL 20 File 4 Name: 1st_EXPERIMENT_-_24hpi_SAL_5_-_44hpi_ROB_20.pcrd File 4 Description: raw qPCR data - first biological replicate (experiment 1)- 24hpi SAL 5, SAL 1 and 44hpi AMP 50, AMP 20, AMP 5, AMP 1, ROB 50, ROB 20 File 5 Name: 1st_EXPERIMENT_-_44hpi_ROB_5_-_52hpi_AMP_20.pcrd File 5 Description: raw qPCR data - first biological replicate (experiment 1)- 44hpi ROB 5, ROB 1, SAL 50, SAL 20, SAL 5, SAL 1 and 52hpi AMP 50, AMP 20 File 6 Name: 1st_EXPERIMENT_-_52hpi_AMP_5_-_SAL_20.pcrd File 6 Description: raw qPCR data - first biological replicate (experiment 1)- 52hpi AMP 5, AMP 1, ROB 50, ROB 20, ROB 5, ROB 1, SAL 50, SAL 20 File 7 Name: 1st_EXPERIMENT_-_52hpi_SAL_5-1___Controls.pcrd File 7 Description: raw qPCR data - first biological replicate (experiment 1)- 52hpi SAL 5, SAL 1 and 24hpi DMEM, 44hpi DMEM, 52hpi DMEM File 8 Name: EXP_2._2hpi_DMSO-ROB_20.pcrd File 8 Description: raw qPCR data - second biological replicate (experiment 2)- 2hpi DMEM, DMSO, AMP 50, AMP 20, AMP 5, AMP 1, ROB 50, ROB 20 File 9 Name: EXP_2._2hpi_ROB_5_-_24hpi_DMEM.pcrd File 9 Description: raw qPCR data - second biological replicate (experiment 2)- 2hpi ROB 5, ROB 1, SAL 50, SAL 20, SAL 5, SAL 1 and 24hpi DMEM File 10 Name: EXP_2._24hpi_AMP_50_-_ROB_1.pcrd File 10 Description: raw qPCR data - second biological replicate (experiment 2)- 24hpi AMP 50, AMP 20, AMP 5, AMP 1, ROB 50, ROB 20 File 11 Name: EXP_2._24hpi_SAL_50_-_44hpi_AMP_20.pcrd File 11 Description: raw qPCR data - second biological replicate (experiment 2)- 24hpi SAL 50, SAL 20, SAL 5, SAL 1 and 44hpi DMEM, AMP 50, AMP 20 File 12 Name: EXP_2._44hpi_AMP_5_-_SAL_20.pcrd File 12 Description: raw qPCR data - second biological replicate (experiment 2)- 44hpi AMP 5, AMP 1, ROB 50, ROB 20, ROB 5, ROB 1, SAL 50, SAL 20 File 13 Name: EXP_2._44hpi_SAL_5_-_52hpi_AMP_1.pcrd File 13 Description: raw qPCR data - second biological replicate (experiment 2)- 44hpi SAL 5, SAL 1 and 52hpi DMEM, AMP 50, AMP 20, AMP 5, AMP 1 File 14 Name: EXP_2._52hpi_ROB_50_-_SAL_1.pcrd File 14 Description: raw qPCR data - second biological replicate (experiment 2)- 52hpi ROB 50, ROB 20, ROB 5, ROB 1, SAL 50, SAL 20, SAL 5, SAL 1 File 15 Name: EXPERIMENT_1.xlsx File 15 Description: The spreadsheets of this file represents each individual plate of the first biological replicate, following the plate design, and contain all raw qPCR data obtained from the CFX Real Time PCR System (Bio-rad Laboratories Inc., USA): Quantification Summary. File 16 Name: EXPERIMENT_2.xlsx File 16 Description: The spreadsheets of this file represents each individual plate of the second biological replicate, following the plate design, and contain all raw qPCR data obtained from the CFX Real Time PCR System (Bio-rad Laboratories Inc., USA): Quantification Summary. METHODOLOGICAL INFORMATION Samples were untreated or treated in duplicated with three different anticoccidial drugs (AMPROLIUM, SALINOMYCIN and ROBENIDIN) at different concentrations (50 µg/ml, 20 µg/ml, 5 µg/ml, and 1 µg/ml) and collected at different times (2, 24, 44 and 52 hours post infection). Two biological replicated were performed. Files are .pcrd to be visualised and processed with Bio-Rad CFX Manager software (Bio-Rad). Data including Ct (Cq) values and standard quantification has been also exported as .xlsx format. The number of parasite for each data point (performed in triplicated) was automatically determined for each well by a regression analysis based in the standard curve (gDNA equivalent to 10e7 followed by serial dilution until 10e1). DATA-SPECIFIC INFORMATION: Abbreviations used: AMP: Sample treated with Amprolium ROB: Sample treated with Robenidine SAL: Sample treated with Salinomycin AMP/ROB/SAL 50: Sample treated with 50 µg/ml of each drug AMP/ROB/SAL 20: Sample treated with 20 µg/ml of each drug AMP/ROB/SAL 5: Sample treated with 5 µg/ml of each drug AMP/ROB/SAL 1: Sample treated with 1 µg/ml of each drug DMEM: Untreated sample DMSO: Sample treated with DMSO 2hpi: Sample collected 2 hours post infection 24hpi: Sample collected 24 hours post infection 44hpi: Sample collected 44 hours post infection 52hpi: Sample collected 52 hours post infection Technical replicates are named as A, B and C.