# Data from: Paternal hatching care regulates the timing, synchrony, and success of hatching in a coral reef fish
## J.E. Majoris, F.A. Francisco, C.M. Burns, S.J. Brandl, K.M. Warkentin, P.M. Buston 

Corresponding Author:
		Name: John E. Majoris
		Institution: University of Texas at Austin, Marine Science Institute
		Address: 750 Channel View Drive, Port Aransas Texas
		Email: john.majoris@austin.utexas.ed

# Metadata:
### All statistical analyses and code were produced in R v. 4.1.0.


### "Figure2_PaternalHatching"
# Folder contains data and code for plotting the age and duration of embryos that were paternally induced to hatch.
- Folder contains: "Figure2_PaternallyCuedHatching_Archive.R", "PaternalHatching.csv", "photoperiod.csv"

## "PaternalHatching.csv"
- File contains the age and hatching duration of paternally induced clutches of embryos. 
 * Parent ID (column: 'PairID')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Time of day when clutch started hatching (column: 'Hatching_time_start')
 * Time of day when clutch stopped hatching (column: 'Hatching_time_stop')
 * Age when clutch started hatching in hours post-fertilization (column: 'Hatch_age_min')
 * Age when clutch stopped hatching in hours post-fertilization (column: 'Hatch_age_max')
 * Duration of hatching in hours (units: Hours ;column: 'Hatch_duration')
 * Percent of embryos that hatched (column: 'Percent_hatched')
 * Sorting variable for visualization (column: 'ID')

## "photoperiod.csv"
- File contains the laboratory lighting conditions.
 * Time of day (1-24) (column: 'Time')
 * Light conditions (0 - off, 1 - on) (column: 'Light')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Embryo age in hours post-fertilization (column: 'AgeHPF')
 * Embryo age in days post-fertilization (column: 'AgeDPF')



### "Figure3_AgeDurationSuccess"
# Folder contains data and code for plotting and statistical analyses assesses the variation in hatching age, duration, and success among treatment groups and times observed.
- Folder contains: "Figure3_AgeDurationSuccess.R", "age.csv", "duration.csv", "success.csv", "MeanProportionHatched.csv"

## "age.csv"
- File contains the age of each larva that hatched from a clutch
 * Experimental treatment (MIH- mechanically-induced hatching, NIH - Non-induced hatching, PCH - Paternally-cued hatching) (column: 'Treatment')
 * Parent ID (column: 'PairID')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Proportion of the embryos in a clutch that hatched (column: 'Hatching_success')
 * Embryo age in hours post-fertilization (column: 'Age')

## "duration.csv"
- File contains the duration over which a clutch hatched
 * Experimental treatment (MIH- mechanically-induced hatching, NIH - Non-induced hatching, PCH - Paternally-cued hatching) (column: 'Treatment')
 * Parent ID (column: 'PairID')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Age in hours post-fertilization when embryos in a clutch began hatching (column: 'min')
 * Age in hours post-fertilization when embryos in a clutch stopped hatching (column: 'max')
 * Duration in hours over which embryos in a clutch hatched (column: 'duration')

## "hsuccess.csv"
- File contains the proportion of a clutch that hatched successfully
 * Experimental treatment (MIH- mechanically-induced hatching, NIH - Non-induced hatching, PCH - Paternally-cued hatching) (column: 'Treatment')
 * Parent ID (column: 'PairID')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Proportion of the embryos in a clutch that hatched (column: 'Hatching_success')
 * Mean hatching age of embryos in a clutch in hours post-fertilization (column: 'mean_Age')
 * Standard deviation of hatching age (column: 'sd_Age')
 * Minimum hatching age of embryos in a clutch in hours post-fertilization (column: 'min_Age')
 * Maximum hatching age of embryos in a clutch in hours post-fertilization (column: 'max_Age')

## "MeanProportionHatched.csv"
- File contains the proportion of embryos that hatched at each 8 hr observation interval by experimental treatment and time observed
 * Experimental treatment (MIH- mechanically-induced hatching, NIH - Non-induced hatching, PCH - Paternally-cued hatching) (column: 'Treatment')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Age of embryos in hours post-fertilization (column: 'Age')
 * Mean number of embryo that hatched in each observation interval (column: 'mean_n')
 * Mean proportion of embryo that hatched in each observation interval (column: 'mean_prop_total')




### "Figure4_HatchlingMorphology"
# Folder contains data and code for plotting and statistical analyses assesses the variation in hatchling morphology among treatment groups and times observed.
- Folder contains: "Figure4_HatchlingMorphology.R", "morphology.csv", "EC_Larvae_FINAL.jpg"

## "morphology.csv"
- File contains the morphological traits of larvae from each clutch
 * Parent ID (column: 'PairID')
 * Experimental treatment (MIH- mechanically-induced hatching, NIH - Non-induced hatching, PCH - Paternally-cued hatching) (column: 'Treatment')
 * Time of day when clutch was first observed (column: 'Time_observed')
 * Age of embryos in hours post-fertilization (column: 'Age')
 * Total length in millimeters (column: 'TL')
 * Propulsive area in square millimeters (column: 'PA')
 * Eye diameter in millimeters (column: 'ED')
 * Yolk sac area in square millimeters (column: 'YA')