The data accompany the article: Cousins-Westerberg, R., Dakin, N., Schat, L., Kadereit, G. & A.M. Humphreys. Evolution of cold tolerance in the highly stress tolerant samphires and relatives (Salicornieae: Amaranthaceae). Bot. J. Linn. Soc. (in review). The data include: The full alignment of all taxa (n=169) as a nexus file. The full Bayesian tree (consensus tree; ntax = 169) as a .tre file. The reduced alignment of one accession per species or subspecies (n=138) as a nexus file. The dated tree generated in Beast, based on the reduced alignment (maximum clade credibility tree; ntax = 138), as a .tre file. A fasta file containing two ITS sequences containing a lot of gaps. A fasta file containing two ITS sequences containing a lot of gaps (with gaps removed).