GENERAL INFORMATION

1. Title of Dataset: Data from: Drivers of intraspecific differentiation of the alpine cold tolerant herb Notopterygium oviforme: the roles of isolation by distance and ecological factors.

2. Author Information
	Corresponding Investigator 
		Name: Dr Mi-Li Liu
		Institution: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi¡¯an 710069, China
		Email: liumili@stumail.mwu.edu.cn

	Co-investigator 1
		Name: Dr Qian-Han Shang
		Institution: State Key Laboratory of Plateau Ecology and Agriculture, College of Ecological and Environmental Engineering, Qinghai University, Xining, 810086, China

	Co-investigator 2
		Name: Mr Yan-Jun Cheng
		Institution: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi¡¯an 710069, China

3. Date of data collection: 2016-2018

4. Geographic location of data collection: Shannxi and Gansu provinces, China

5. Funding sources that supported the collection of the data: Northwest University

6. Recommended citation for this dataset: Liu, Mili (2022), Drivers of intraspecific differentiation of the alpine cold tolerant herb Notopterygium oviforme: the roles of isolation by distance and ecological factors, Dryad, Dataset, 


DATA & FILE OVERVIEW

1. Description of dataset
These data were generated to 11 natural wild populations (191 individuals) of N. oviforme across the whole geographic distribution of  in the Qinling Mountains and adjacent areas. In the field, the distance between any two sampled individuals was at least 50 m. Some fresh leaves were also dried with silica gel prior to DNA extraction. The purity and integrity of DNA were evaluated using agarose gel electrophoresis. 15 polymorphic simple sequence repeat (SSR) markers were selected to amplify 191 individuals of N. oviforme. The amplified products were quantified using gel electrophoresis and sent to Tsingke Company (Xian, Shannxi, China) for capillary separation analysis. 

2. File List: 
	File 1 Name: data.xlsx

	
METHODOLOGICAL INFORMATION

Fifteen polymorphic simple sequence repeat (SSR) markers were selected to amplify 191 individuals of N. oviforme. The amplified products were quantified using gel electrophoresis and sent to Tsingke Company (Xian, Shannxi, China) for capillary separation analysis. For each microsatellite locus, we detected the potential loci under selection using Arlequin v3.5 software (Excoffier & Lischer, 2010), and these possibly selected loci were removed for subsequent population genetic analysis. 

SSR primer sequences and annealing temperature.

	Primer	Primer sequences (5¡ä-3¡ä)	Annealing Temperature (¡ãC)
	17078	F: AGGTGGAAATGTCAAGTGGC	56
		R: CACCGTCCTCTGCTTCTTTC	
	15156	F: CGCAGCAACAACAACAAACT	55
		R: TAAGTAGCCGGAGCCTTGTC	
	16151	F: TTTAAGGAATGACCAACGGC	57
		R: AGTGGAGGAGGATCATGGTG	
	18934	F: GCCATCACTAGCCTTTGGAG	54
		R: GTCGCTTTGCCCGTAAATAA	
	15648	F: TTGTGAGGAATTAGCTGGGG	56
		R: GGGTCCAGTCTCAGCTACCA	
	14323	F: CCCAACAC CACCAAACAAG	55
		R: GACGCTGCAAAGGAAATGTT	
	15567	F: GATCTCAGATGAGGAAGCGG	55
		R: TCAACGCTCGATTTCATCAG	
	14926	F: TTGCTTCGACGAATCCATAG	55
		R: GGCAAAACAATCCTCAGTCAA	
	12542	F: TTGTCTTCTGCGGTTGTACG	55
		R: ATTCTCCTTCTCTCCGCCAT	
	16678	F: ACATGTCCTCCATGGACTGG	54
		R: GCCTAGTAAGCGTCGTCGTC	
	19210	F: GTTGTGGAGGAGAAGGCAAA	55
		R: CAACTGTCTCCTCCGCTTTC	
	14424	F: GTGAATGTTGTGGACCTCCC	55
		R: GGCAGGATACGAAGCCTAGA	
	15401	F: GAGGGTTTTACTGCAGAGACAGA	54
		R: TCGCACTCAATCATGCTACC	
	18018	F: CCATGGCCTTTGCTCATTAC	54
		R: CGATGAAGAAGCTTGGGAAA	
	14072	F: GGTCGGGTCTCACACAAAAT	55
		R: TGGATACTTTTTCTGTTTGGTCTTT	

1. Number of variables: 15

2. Number of cases/rows: 191

3. Variable List: 
	19210: Polymorphic simple sequence repeat markers (SSR)
	14424: Polymorphic simple sequence repeat markers (SSR)
	15401: Polymorphic simple sequence repeat markers (SSR)
	18018: Polymorphic simple sequence repeat markers (SSR)
	17078: Polymorphic simple sequence repeat markers (SSR)
	14072: Polymorphic simple sequence repeat markers (SSR)
	15156: Polymorphic simple sequence repeat markers (SSR)
	16151: Polymorphic simple sequence repeat markers (SSR)
	18934: Polymorphic simple sequence repeat markers (SSR)
	15648: Polymorphic simple sequence repeat markers (SSR)
	14323: Polymorphic simple sequence repeat markers (SSR)
	15567: Polymorphic simple sequence repeat markers (SSR)
	14926: Polymorphic simple sequence repeat markers (SSR)
	12542: Polymorphic simple sequence repeat markers (SSR)

4. Missing data codes: 
	0

5. Abbreviations used: 
	This is not

6. Other relevant information: 
	The number size represent the bases number.