{"text_format":"3","workbook":"xls","n_sheets":4,"sheets":["Metadata","Matrix non-normalized","Matrix normalized","Fold Change"],"not_rendered":{"external_links":1,"comments":22},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Metadata","index":0,"n_rows":47,"n_cols":9,"rows_kept":47,"merged":[[1,1,1,5],[2,1,3,8],[4,1,4,10],[17,1,17,10],[18,1,18,10],[19,1,19,10]],"flags":["formulas_cached_only"]}
["SERIES"]
["title","Real-time quantitative PCR analysis of differentiated C6 astrocyte-like cells"]
["summary","C6 cells were grown on PCL-membranes functionalized with different graphene-based nanomaterials. Cells were differentiated to astrocytes after 5 days of dbcAMP treatment (1mM) in low serum (0.5%) DMEM."]
["summary"]
["overall design","qPCR gene expression profiling.  dbcAMP-treated C6 astrocytes from 2-5 experiments were used. Equal amounts of total RNA (1µg) from each experiment were used independently to analyze gene expression. "]
["contributor","Marián, Mantecón-Oria"]
["contributor","Olga, Tapia"]
[]
["SAMPLES"]
["Sample name","title","description","source name","organism","characteristics: nanomaterial","molecule","label","platform"]
["SAMPLE 1","PCL","Control","Differentiated C6 cells","rat","Control ","total RNA","SYBR Green"]
["SAMPLE 2","PCL/Gmec","test","Differentiated C6 cells","rat","Gmec","total RNA","SYBR Green"]
["SAMPLE 3","PCL/Ganodic","test","Differentiated C6 cells","rat","Ganodic","total RNA","SYBR Green"]
["SAMPLE 4","PCL/rGO","test","Differentiated C6 cells","rat","rGO","total RNA","SYBR Green"]
["SAMPLE 5","PCL/GO","test","Differentiated C6 cells","rat","GO","total RNA","SYBR Green"]
[]
["PROTOCOLS"]
["growth protocol","C6 cells were cultured in DMEM enriched with 10% FBS, 1% NEAA and 1% P/S at 37oC in humid atmosphere containing 5% CO2."]
["treatment protocol","C6 cells were cultured for 5 days in DMEM enriched with 0.5% FBS, 1% NEAA and 1% P/S at 37oC in humid atmosphere containing 5% CO2, containing 1mM of dbcAMP."]
["extract protocol","Total RNA was extracted with TRIZOL (Invitrogen) followed by purification with RNeasy Kit (QIAGEN)"]
["label protocol","Reverse transcription was performed from 150 ng total RNA using the High Capacity cDNA reverse Transcription kit (Life Technologies). PCR assays were performed using LightCycler 480 SYBR Green I Master as recomended by the manufacturer."]
["hyb protocol","n/a"]
["scan protocol","n/a"]
["data processing","Raw threshold cycle data was normalized using the housekeeping gene GAPDH. DeltaCt was calculated according to the formula: (Ct_Target − Ct_GAPDH)"]
["data processing","2^-deltaCt target gene signals normalized to housekeeping genes were calculated."]
["data processing","DeltadeltaCt based fold-change were calculated and represented in a bar diagram."]
["value definition","Matrix normalized worksheet reports normalized signal (against housekeeping gene GAPDH)."]
["value definition","Fold Change worksheet reports test/control (i.e., (PCL/Gmec) / PCL) ratios."]
[]
["PLATFORM"]
[]
["title"]
["technology"]
["distribution"]
["organism"]
["manufacturer"]
["manufacture protocol"]
["catalog number"]
["description"]
["description"]
[]
["# Platform Column Definitions: describe the contents of each platform column in the Matrix normalized Template"]
["header 1","UniGene cluster ID"]
["header 2","RefSeq accession number of spotted sequence"]
["header 3","Gene symbol"]
["header 4","Gene description"]
["header 5","Gene name"]

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["ID_REF","SAMPLE 1","SAMPLE 2","SAMPLE 3","SAMPLE 4","SAMPLE 5"]
["A01  GFAP",{"v":30.192461013793945,"d":"30.19"},{"v":28.044191360473633,"d":"28.04"},{"v":27.965778350830078,"d":"27.97"},{"v":27.427799224853516,"d":"27.43"},{"v":27.22125244140625,"d":"27.22"}]
["A02 ",{"v":30.128522872924805,"d":"30.13"},{"v":29.715808868408203,"d":"29.72"},{"v":28.264482498168945,"d":"28.26"},{"v":27.736539840698242,"d":"27.74"},{"v":27.163631439208984,"d":"27.16"}]
["A03 ",{"v":29.980316162109375,"d":"29.98"},26.88,26.13,{"v":27.659761428833008,"d":"27.66"},25.57]
["A04 ",28.27,"-",25.98,25.57,25.41]
["A05 ",28.26,"-","-",25.44,"-"]
["A06 Glast",36.56,35.23,{"v":35.6,"d":"35.60"},34.58,33.24]
["A07",36.18,35.57,34.64,33.81,33.39]

{"sheet":"Matrix normalized","index":2,"n_rows":8,"n_cols":11,"rows_kept":8,"flags":["formulas_cached_only"]}
["ID_REF","Unigene,","GB_ACC ","Symbol","Description","Gene name","SAMPLE 1","SAMPLE 2","SAMPLE 3","SAMPLE 4","SAMPLE 5"]
["A01  ","Rn.91512","NM_017009 ","Gfap","Glial fibrillary acidic protein","Gfap",0.002359404218557405,0.0034647918649221322,0.005933863934431276,0.0055366137895474215,0.005457948370725982]
["A02 ","Rn.91512","NM_017009 ","Gfap","Glial fibrillary acidic protein","Gfap",0.0025521572220314915,0.0027507295150777608,0.005815986882987082,0.006182536429406928,0.005780792859791558]
["A03 ","Rn.91512","NM_017009 ","Gfap","Glial fibrillary acidic protein","Gfap",0.002409277672409942,0.0033075988764161247,0.006129563264818374,0.005476647625325351,0.005118724233821736]
["A04 ","Rn.91512","NM_017009 ","Gfap","Glial fibrillary acidic protein","Gfap",0.0022906932602185155,"-",0.006801176275750972,0.005013382412355095,0.006087223278597658]
["A05 ","Rn.91512","NM_017009 ","Gfap","Glial fibrillary acidic protein","Gfap",0.002243551474603584,"-","-",0.005410583859808297,"-"]
["A06 ","Rn.34134","NM_019225","Slc1a3","Solute carrier family 1 member 3 (glial high affinity glutamate transporter)","Slc1a3/Glast",7.31860442600451e-06,1.0137068283817414e-05,8.643218119017016e-06,1.1170087401928924e-05,2.513400321251139e-05]
["A07","Rn.34134","NM_019225","Slc1a3","Solute carrier family 1 member 3 (glial high affinity glutamate transporter)","Slc1a3/Glast",9.263571810875302e-06,8.348798379980002e-06,1.1889107966011061e-05,1.6353965187626536e-05,2.4110147456768107e-05]

{"sheet":"Fold Change","index":3,"n_rows":8,"n_cols":5,"rows_kept":8,"flags":["formulas_cached_only"]}
["ID_REF","SAMPLE 2/SAMPLE 1","SAMPLE 3/SAMPLE 1","SAMPLE 4/SAMPLE 1","SAMPLE 5/SAMPLE 1"]
["A01",1.4613105691188297,2.5026663710699815,2.3351221554477224,2.3019442294915518]
["A02 ",1.1601476423059494,2.4529505474801465,2.6075464791180405,2.438107116743048]
["A03 ",1.3950128564565525,2.5852045179524556,2.309830826853242,2.1588730621937353]
["A04 ","-",2.8684640121719105,2.114444097026187,2.567347205948501]
["A05 ","-","-",2.281967774020766,"-"]
["A06 ",1.2226463087844806,1.0424709031609436,1.347240222557271,3.0314480866041906]
["A07",1.0069605172102496,1.4339623214917874,1.9724751388486972,2.907959379076627]