DMAN: Differential scanning fluorimetry melting data analysis

DMAN is a program to analyse protein thermal denaturation data obtained by multi-well differential scanning fluorimetry. Experimental repetition schemes on multi-well plates can be defined using an ASCII file or through a graphical user interface (GUI). The raw or pre-processed protein denaturation data are read by DMAN, and results of replica wells are averaged with the mean melting temperature and the estimate of standard deviation being calculated. Outliers in a set of replica are automatically excluded based on Grubbs' test using a probability threshold of 95%. The results are annotated to highlight wells that show a significant difference in melting temperature from a reference well, as well as the p-value for this difference determined by Welch's t-test. The melting curves and the location of determined inflection points can be graphically displayed for each set of replica; the user can also determine the inflection point interactively.

When using this program, please cite:

Wang, C.K., Weeratunga, S.K., Pacheco, C.M., Hofmann, A. (2012) DMAN: a Java tool for analysis of multi-well differential scanning fluorimetry experiments. Bioinformatics 28, 439-440.