This BLKI.2017.withdrawal.expt.readme.txt file was generated on 2022-06-23 by Z Morgan Benowitz-Fredericks GENERAL INFORMATION 1. Title of Dataset: Black-legged kittiwake physiology & behavior: 2017 Food withdrawal experiment 2. Author Information A. Principal Investigator Contact Information Name: Z Morgan Benowitz-Fredericks Institution: Bucknell University Address: Dept. of Biology, 1 Dent Dr., Lewisburg PA 17837 Email: zmbf001@bucknell.edu B. Associate or Co-investigator Contact Information Name: Alexander Kitaysky Institution: Institute of Arctic Biology, Dept. of Biology and Wildlife, Univ. Alaska Fairbanks Email: akitaysky@alaska.edu 3. Date of data collection (single date, range, approximate date): 2017-06-18 - 2017-07-10 4. Geographic location of data collection: Middleton Island, AK (59.4283° N, 146.3300° W) 5. Information about funding sources that supported the collection of the data: Funding came from a National Science Foundation ADVANCE grant (HRD-1464048; ZMBF), a Sigma Xi Grant-in-Aid-of-Research (G2017031593424085, L.M.L.), IAB UAF, Alaska INBRE support to the UAF Core lab (A.S.K); the Natural Sciences and Engineering Research Council (S.W.) and U.S. Geological Survey via Gulf Watch Alaska (Exxon-Valdez Oil Spill Trustee Council). SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: Please contact PI before using 2. Links to publications that cite or use the data: Not yet available - in press @ ProcB 3. Links to other publicly accessible locations of the data: N/A 4. Links/relationships to ancillary data sets: N/A 5. Was data derived from another source? NO DATA & FILE OVERVIEW 1. File List: File #1- BenowitzFredericks et al BLKI Food Withdrawal Study 2017 - Data for all analyses EXCEPT for Supplemental Figure 1 (Age v. telomere length for chicks and adults) File #2- BenowitzFredericks et al BLKI Age Telo 2017 - Data for Supplemental Figure 1 (Age v. telomere length for chicks and adults) 2. Relationship between files, if important: File #1 contains data for all analyses in study except age v. telomeres, which is in file #2 3. Additional related data collected that was not included in the current data package: Raw GPS data not included 4. Are there multiple versions of the dataset? No METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: Detailed methods in Benowitz-Fredericks et al. 2022, Proceedings of the Royal Society of London, Series B (open access) 2. Methods for processing the data: Raw GPS data were converted into "proportion of time in State 1" and "proportion of time in State 2" as described in paper methods. 3. Instrument- or software-specific information needed to interpret the data: All data were analyzed in R (Version 3.4.1). Code (including relevant packages/libraries) is included in the electronic supplemental material associated with the publication (open access). 4. Standards and calibration information, if appropriate: N/A 5. Environmental/experimental conditions: Nests received supplemental fish 3x/day beginning in May 2017. Experimental birds had the food supplemental withdrawn after 48 of GPS tracking. Controls continued to receive the food supplement. All birds were bled at first capture, and again ~96hr later (48 hrs after withdrawal). Details in paper methods. 6. Describe any quality-assurance procedures performed on the data: Data were proofed in the field Corticosterone and telomere samples were run in replicate, with assaying lab blind to treatments Data were check for normality and transformed as needed (see paper methods) 7. People involved with sample collection, processing, analysis and/or submission: Experimental design & data submission: Z Morgan Benowitz-Fredericks Sample collection: Z Morgan Benowitz-Fredericks, Lindsay Mae Lacey GPS data processing/analysis: Shannon Whelan, Alexis Will Corticosterone and telomere assays: Alexander Kitaysky DATA-SPECIFIC INFORMATION FOR: BenowitzFredericks et al BLKI Food Withdrawal Study 2017 1. Number of variables: 29 2. Number of cases/rows: 20 3. Variable List (and units): BirdID = unique color band code from Middleton island Missing.data = which variables are missing for a given individual nest.ID = nest site on Middleton Island 2017 Treatment = continuously food supplemented ("control") or 48 of food withdrawal ("withdrawal") Focal Bird Band # = USFWS permanent metal band # Year.first.banded = when first banded on Middleton known.age = age at capture if banded as nestling on Middleton island min.age.plus7 = known age if first banded on Middleton island as nestling, year first banded + 7 if first captured as an adult on Middleton relative.telo.length = relative telomere length (from RT-qPCR - see methods) qPCR.plate.final.telo = plate number for telomere assay BaseCORT = baseline plasma corticosterone (in ng/mL) at first capture (when all birds food supplemented; blood taken in <3min from initial disturbance)) log10.BaseCORT = log transformed baseline plasma corticosterone from first sampling FinalCORT = baseline plasma corticosterone ~96 hrs after initial blood sample (48hrs after food withdrawal for withdrawal birds, same duration no withdrawal for control birds, blood taken in <3min after capture) deltaCORT = final baseline plasma corticosterone - initial baseline plasma corticosterone (change from pre- to post- withdrawal). logdCORT = log10 transformed change in corticosterone massStart = body mass (in grams) at first capture massEnd = body mass (in grams) at second capture masschange = body mass at second capture - body mass at first capture 2017 # eggs = # of eggs during incubation in study year 2017 # eggs hatched = # eggs that hatched during study year chicks.fledged.2017 = number of chicks that fledged during study year 2017 at least 1 chick = fledged at least one chick = 1, did not fledge a chick = 0 Returned 2018 = was relighted at the colony in 2018 = 1, was not resighted at the colony in 2018 (or in 3 subsequent years) = 0 Pre.Prop.State1 = proportion of time spent in "state 1" movement behavior in 1st 48 hrs, as determined with a 2-state Hidden Markov model Post.Prop.State1 = proportion of time spent in "state 1" movement behavior in 2nd 48 hrs (post food withdrawal for withdrawal birds), as determined with a 2-state Hidden Markov model Pre.Prop.State2 = proportion of time spent in "state 2" movement behavior in 1st 48 hrs, as determined with a 2-state Hidden Markov model Post.Prop.State2 = proportion of time spent in "state 1" movement behavior in 2nd 48 hrs (post food withdrawal for withdrawal birds), as determined with a 2-state Hidden Markov model Change.Prop.State1 = change in proportion of time spent in State 1 movement from first to 2nd 48 hrs Change.Prop.State2 = change in proportion of time spent in State 2 movement from first to 2nd 48 hrs 4. Missing data code: n/a Some data points are missing due to failure to collect a timely blood sample or failed GPS units DATA-SPECIFIC INFORMATION FOR: BenowitzFredericks et al BLKI Age Telo 2017 1. Number of variables: 7 2. Number of cases/rows: 54 3. Variable List (and units): Nest.ID = location of nest on Middleton Island tower (2017 for adults, 2016 for chicks) known age (if hatched/banded on tower) = only for birds first banded as chicks on Middleton Min age (if captured AHY, years since 1st captured + 7) = known or estimated age age.method = how age was determined relative telomere length = relative telomere length as determined by quantitation real time PCR using frozen red blood cells Age = whether adult (variable ages) or chick (all chicks sampled at exactly 10 days post hatch in 2016) qPCR Assay for telo = which plate/assay for telomere analysis 4. Missing data code: n/a Data points are missing in the known age column for adults who were not first banded on the tower as chicks