Preliminary phytochemical screening of methanolic extract of Azadirachta Indica and antibacterial activities against foot borne and contaminated bacteria

Wound and food borne causes an increasing burden to healthcare systems as the availability of drugs capable of stimulating the process of wound repair is still limited in order to treat the patient. In addition, the increasing prevalence of antibiotic resistance bacteria is widely recognized and poses a continuing burden to healthcare. Therefore, the need of novel antibiotics for effective treatment for wound infection is necessary in order to eliminate the threat of the infections. Objective of this study was to investigate the in vitro antibacterial activity of medicinal plant, Azadirachta indica or its local name ‘neem’, on some selected infectious bacterias. Methanolic Azadirachta Indica extract was used to test on the bacteria at five different concentrations. Antimicrobial susceptibility testing (AST) were used in this study. Zone of inhibition have been shown to Staphylococcus aureus, Pseudomonas aeruginosa and E.coli and were considered significant. This study showed that methanolic Azadirachta Indica extract possess antimicrobial activity and further studies need to be done in order to exploit the bioactive compounds of Azadirachta Indica in order to reveal its potential as a new source of antibacterial agent.