Supporting files for:
Mechanisms of isothiocyanate detoxification in larvae of two belowground herbivores, Delia radicum and D. floralis (Diptera: Anthomyiidae)
Rebekka Sontowski*, Cervin Guyomar, Yvonne Poeschl, Alexander Weinhold, Nicole M. van Dam and Daniel G. Vassão*

*corresponding author: Rebekka Sontowski (rebekka.sontowski@idiv.de) and Daniel G. Vassão (vassao@ice.mpg.de)
Please dont hesitate to contact the corresponding author when ypu need any further information.

If you use any of these data, please cite the corresponding DOI and the manuscript Sontowski et al. 2022.

We provide additional data like genome sequences, gene annotation, functional annotation for D. floralis we used to generate results described in our manuscript. We also provide resluting DESeq2 counts including log2-fold changes and corresponding statistics.
In addition we provide intermediate results on the assembly and annotation of unmapped reads.

We provide:

genome sequences and gene annotation in:
genome_and_annotation.zip
	containing:	iDiv_DFl_1.0.fasta
				iDiv_DFl_1.0.gff
				t2g_DFl.csv

functional annotation in:
functional_annotation.zip
	containing:	PANNZER_geneIDs.txt

gene expresion in:
gene_expression.zip
	containing: 
		DESeq2 counts and statistics:
				counts_test_stats_expressed_genes.xlsx
				counts_test_stats_expressed_genes.csv
				condition_floralis_ITC_vs_floralis_control_de_genes_sign_with_GO.tsv
				condition_radicum_ITC_vs_radicum_control_de_genes_sign_with_GO.tsv
		list of expressed genes:
				radicum_floralis_expressed_genes.txt
				euler_venn_expressed_genes.pdf


unmapped reads assembly and further processing, and intermediate annotations in:
unmapped_reads_assembly_and_processing_annotation.zip
	containing:
		Annotation from the lift-over of D. radicum to D. floralis by liftover:
				radicum_to_floralis_liftoff.gff
		Trinity assembly of unmapped reads:
				Trinity.fasta
		Transdecoder annotation:
				transcripts.fasta.transdecoder.gff3
		final annotation after processing with GeMoMa's AnotationEvidence, GAF, and AnnotationFinalizer and adding an offset to the geneIDs:
				final_trinity_annotation_addOffSet2GeneNumbers.gff
		filtered Trinity sequences based on final annotation:
				Trinity_filtered.fasta