Refer to the ReadMe files for information on the included data and script files.
\nmicrocolony_ReadMe.txt provides details on the microcolony response datasets.
\nsequences_ReadMe.txt provides information on how to access raw sequences and on sample data associated with 16S and ITS phyloseq objects.
\nScript_ReadMe.txt provides information on the R scripts used to analyze data associated with this project.
", "type": { "id": "notes", "title": { "de": "Anmerkungen", "en": "Notes" } } } ], "creators": [ { "affiliations": [ { "name": "University of California, Davis" } ], "person_or_org": { "family_name": "Rutkowski", "given_name": "Danielle", "identifiers": [ { "identifier": "0000-0001-6156-1280", "scheme": "orcid" } ], "name": "Rutkowski, Danielle", "type": "personal" } }, { "affiliations": [ { "name": "University of California, Davis" } ], "person_or_org": { "family_name": "Litsey", "given_name": "Eliza", "name": "Litsey, Eliza", "type": "personal" } }, { "affiliations": [ { "name": "University of California, Davis" } ], "person_or_org": { "family_name": "Maalouf", "given_name": "Isabelle", "name": "Maalouf, Isabelle", "type": "personal" } }, { "affiliations": [ { "name": "University of California, Davis" } ], "person_or_org": { "family_name": "Vannette", "given_name": "Rachel", "identifiers": [ { "identifier": "0000-0002-0447-3468", "scheme": "orcid" } ], "name": "Vannette, Rachel", "type": "personal" } } ], "description": "Bumble bees are important pollinators that face threats from multiple sources, including agrochemical application. Declining bumble bee populations have been linked to fungicide application, which could directly affect the fungi often found in the stored food and gastrointestinal (GI) tract of healthy bumble bees.
\nWe test the hypothesis that fungicides impact bee health by disrupting bee\u2013fungi interactions. We examined the interactive effects of the fungicide propiconazole and fungal supplementation on the survival, reproduction and microbiome composition of microcolonies (queenless colonies) using two species,\u00a0Bombus vosnesenskii\u00a0and\u00a0B. impatiens.
\nWe found that in\u00a0B. vosnesenskii, fungicide exposure decreased survival, while fungal supplementation mitigated fungicide effects. For\u00a0B. impatiens, fungicide application had no effect, but fungal supplementation improved survival and offspring production.
\nFungicides reduced fungal abundance in\u00a0B. vosnesenskii\u00a0microcolonies, but not in\u00a0B. impatiens, where instead fungal addition decreased fungal abundance (ITS copy number). Fungal composition varied between treatments but differently between bee species. In\u00a0B. impatiens, fungal addition increased microbiome diversity. In\u00a0B. vosnesenskii, the abundance of the pathogen\u00a0Ascosphaera\u00a0was negatively associated with survival, while the yeast\u00a0Zygosaccharomyces\u00a0was positively associated with survival.
\nOur results highlight that bumble bee species differ in response to fungicides and in the nature of bee\u2010fungi associations. Fungicides can alter bee\u2013fungi interactions with consequences for bee survival and reproduction, and exploring the mechanisms of such interactions, including interactions among fungi in the bee GI tract, may offer insights into bumble bee biology and conservation strategies.
", "publication_date": "2022-02-07", "publisher": "Zenodo", "related_identifiers": [ { "identifier": "10.1101/2021.09.06.459164", "relation_type": { "id": "iscitedby", "title": { "de": "Wird zitiert von", "en": "Is cited by" } }, "scheme": "doi" }, { "identifier": "10.5281/zenodo.5484180", "relation_type": { "id": "isderivedfrom", "title": { "de": "Wird abgeleitet von", "en": "Is derived from" } }, "scheme": "doi" } ], "resource_type": { "id": "dataset", "title": { "de": "Datensatz", "en": "Dataset" } }, "rights": [ { "description": { "en": "CC0 waives copyright interest in a work you've created and dedicates it to the world-wide public domain. Use CC0 to opt out of copyright entirely and ensure your work has the widest reach." }, "icon": "cc-cc0-icon", "id": "cc0-1.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/publicdomain/zero/1.0/legalcode" }, "title": { "en": "Creative Commons Zero v1.0 Universal" } } ], "subjects": [ { "subject": "Ascosphaera" }, { "subject": "bee microbiome" }, { "subject": "Bombus" }, { "subject": "propiconazole" }, { "subject": "starmerella" }, { "subject": "yeast" } ], "title": "Bee-associated fungi mediate effects of fungicides on bumble bees" }, "parent": { "access": { "owned_by": { "user": "90070" } }, "communities": { "default": "734b6452-1f40-4db0-b218-08ab1d568b7f", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_submission_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": false }, "created": "2020-02-04T14:03:17.992421+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "734b6452-1f40-4db0-b218-08ab1d568b7f", "links": {}, "metadata": { "curation_policy": "The Dryad collection in Zenodo contains datasets mirrored from the Dryad Digital Repository. If you want your dataset to appear here, submit it through https://datadryad.org. No other datasets will be accepted.
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