This readme.txt file was generated on 2021-01-28 by OMERA MATOO

GENERAL INFORMATION

1. Title of Dataset: Phenotypic data for M.edulis responses to warming and ocean acidification

2. Author Information
	A. Principal Investigator Contact Information
		Name: Omera B Matoo
		Institution:University of Nebraska-Lincoln
		Address: 315, Manter Hall, School of Biological Sciences, 1101 T street, University of Nebraska-Lincoln,NE, 68588 
		Email: omatoo2@unl.edu

	B. Associate or Co-investigator Contact Information
		Name: Gisela Lannig
		Institution:Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research 
		Address:D-27570,Bremerhaven, Germany 
		Email: gisela.lannig@awi.de
        
		Name:Christian Bock 
		Institution:Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research 
		Address:D-27570,Bremerhaven,Germany 
		Email:christian.bock@awi.de

                Name:Inna M. Sokolova 
		Institution:University of Rostock 
		Address:18051, Rostock, Germany
		Email:inna.sokolova@uni-rostock.de


3. Date of data collection (single date, range, approximate date):2011-04-01 to 2013-12-15 

4. Geographic location of animal collection Biddeford Pool, Gulf of Maine (43°26'50.6N, 70°21'19.0W).  

5. Information about funding sources that supported the collection of the data: US National Science Foundation (NSF) award IOS-1557870 to Inna M. Sokolova.


DATA & FILE OVERVIEW

1. File List: 
<list all files (or folders, as appropriate for dataset organization) contained in the dataset, with a brief description>

2. Relationship between files, if important: 

3. Additional related data collected that was not included in the current data package: 

4. Are there multiple versions of the dataset? yes/no
	A. If yes, name of file(s) that was updated: 
		i. Why was the file updated? 
		ii. When was the file updated? 


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data:
The phenotypic data for assays was collected using previously published protocols: metabolic rates (Matoo et al., 2013), tissue and cellular energy reserves(Ivanina et al., 2013), enzyme activities (Ivanina et al., 2013, Ivanina et al., 2020) and NMR-based metabolomics (Lannig et al., 2010; Dickinson et al., 2012).     

2. People involved with sample collection, processing, analysis and/or submission:
Omera Matoo, Gisela lannig, Christian Bock and Inna Sokolova.  


DATA-SPECIFIC INFORMATION FOR: [STANDARD METABOLIC RATE]

1. Number of variables:7 

2. Number of cases/rows: 33

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),ß O2 (µM/Torr),Biological Replicate, Dry Tissue weight(g),MO2 (µmol O2/g/h)

DATA-SPECIFIC INFORMATION FOR: [LIPIDS]

1. Number of variables:5 

2. Number of cases/rows: 25

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate, Total Lipids(g/g wet tissue) 

DATA-SPECIFIC INFORMATION FOR: [GLYCOGEN]

1. Number of variables:5 

2. Number of cases/rows: 40

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate, Adductor muscle glycogen (µmol/g)

DATA-SPECIFIC INFORMATION FOR: [ADENYLATES]

1. Number of variables:6 

2. Number of cases/rows: 73

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate, Adenylate(ATP/ADP/AMP), Adenylate (umol g-1)

DATA-SPECIFIC INFORMATION FOR: [AEC]

1. Number of variables:5 

2. Number of cases/rows: 25

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate, AEC

DATA-SPECIFIC INFORMATION FOR: [H+ -ATPase]

1. Number of variables:5 

2. Number of cases/rows: 22

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate,H+ ATPase activity (µmol of Pi/µg protein/ hr)

DATA-SPECIFIC INFORMATION FOR: [Ca2+ -ATPase]

1. Number of variables:5 

2. Number of cases/rows: 24

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate,Ca2+ ATPase activity (µmol of Pi/µg protein/ hr)

DATA-SPECIFIC INFORMATION FOR: [CARBONIC ANHYDRASE]

1. Number of variables:5 

2. Number of cases/rows: 25

3. Variable List:
Species, PCO2 (µatm),Temperature (ºC),Biological Replicate,Specific CA, U/g protein

DATA-SPECIFIC INFORMATION FOR: [TEMPERATURE CHARACTERIZATION OF CARBONIC ANHYDRASE]

1. Number of variables:8

2. Number of cases/rows: 336

3. Variable List:
Organism, Sample name,Tissue,Nominal temperature, C,Aver. CA (U g-1 wet mass), Aver. esterases (U g-1 wet mass),Specific CA (U g protein),Specific esterases (U g-1 protein).

DATA-SPECIFIC INFORMATION FOR: [METABOLOMICS]

1. Number of variables:26

2. Number of cases/rows: 52

3. Variable List:
Adductor 10_395	Group	Acetate	Acetoacetate	Alanine	Arginine	Aspartate	Betaine	Carnitine	Choline	Dimethylamine	Dimethyl sulfone	Glucose	Glutamate	Glutamine	Glycine	Isoleucine	Lactate	Leucine	O-Phosphocholine	Serine	Succinate	Taurine	Trimethylamine N-oxide	Tyrosine	Valine

DATA-SPECIFIC INFORMATION FOR: [WATER CHEMISTRY]

1. Number of variables:11

2. Number of cases/rows:248

3. Variable List:salnity (PSU)	Nominal Temperature (ºC)	Nominal PCO2 (µatm)	Achieved Temperature (ºC) 	pH (NBS)	pCO2 (μatm)	HCO3 (μmol kg-1 SW)	CO3 (μmol kg-1 SW)	CO2 (aq) (μmol kg-1 SW)	Ω calcite 	Ω aragonite