Interpretative toolkit to enable the use of genomic data aligned with physiological status for representative mixoplankton.

Executive Summary
 In Prymnesum parvum (CM), Prymnesin production and the expression of the biosynthesis pathway associated polyketide synthases have been studied and metabolic trade-offs have been elucidated with transcriptomics.
 Mixotrophy in the kleptoplastidic and karyokleptic Strombidium cf basimorphum (GNCM) has been studied and detailed by a combinational approache of single-cell transcriptomics, qPCR analysis and fluorescence in-situ hybridisation (FISH).
 The combined approach of qPCR and FISH appears to be a promising tool to investigate the potential retention of prey nuclei in NCM.
 Culture based studies allow to take samples of organisms with a known feeding history making easier the link of physiology and genomic.
 Metabolic interactions of symbiont and Acantharia (eSNCM, host) of endosymbiotic mixoplankton can be explored using single-cell transcriptomics, and linked to single-cell physiological approaches (such as NanoSIMS).