Published March 5, 2016 | Version v1
Journal article Open

Automatic segmentation of myocardium at risk from contrast enhanced SSFP CMR: validation against expert readers and SPECT

  • 1. Department of Clinical Physiology, Skåne University Hospital in Lund, Lund University, Lund, Sweden
  • 2. Department of Cardiology, Henri Mondor Hospital, Creteil, France
  • 3. Department of Cardiology, Lund University, Lund, Sweden
  • 4. Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
  • 5. Department of Cardiology B, Oslo, University Hospital Ullevål and Faculty of Medicine, University of Oslo, Oslo, Norway

Description

Background: Efficacy of reperfusion therapy can be assessed as myocardial salvage index (MSI) by determining the size of myocardium at risk (MaR) and myocardial infarction (MI), (MSI = 1-MI/MaR). Cardiovascular magnetic resonance (CMR) can be used to assess MI by late gadolinium enhancement (LGE) and MaR by either T2-weighted imaging or contrast enhanced SSFP (CE-SSFP). Automatic segmentation algorithms have been developed and validated for MI by LGE as well as for MaR by T2-weighted imaging. There are, however, no algorithms available for CE-SSFP. Therefore, the aim of this study was to develop and validate automatic segmentation of MaR in CE-SSFP.

Methods: The automatic algorithm applies surface coil intensity correction and classifies myocardial intensities by Expectation Maximization to define a MaR region based on a priori regional criteria, and infarct region from LGE. Automatic segmentation was validated against manual delineation by expert readers in 183 patients with reperfused acute MI from two multi-center randomized clinical trials (RCT) (CHILL-MI and MITOCARE) and against myocardial perfusion SPECT in an additional set (n = 16). Endocardial and epicardial borders were manually delineated at end-diastole and end-systole. Manual delineation of MaR was used as reference and inter-observer variability was assessed for both manual delineation and automatic segmentation of MaR in a subset of patients (n = 15). MaR was expressed as percent of left ventricular mass (%LVM) and analyzed by bias (mean ± standard deviation). Regional agreement was analyzed by Dice Similarity Coefficient (DSC) (mean ± standard deviation).

Results: MaR assessed by manual and automatic segmentation were 36 ± 10 % and 37 ± 11 %LVM respectively with bias 1 ± 6 %LVM and regional agreement DSC 0.85 ± 0.08 (n = 183). MaR assessed by SPECT and CE-SSFP automatic segmentation were 27 ± 10 %LVM and 29 ± 7 %LVM respectively with bias 2 ± 7 %LVM. Inter-observer variability was 0 ± 3 %LVM for manual delineation and -1 ± 2 %LVM for automatic segmentation.

Conclusions: Automatic segmentation of MaR in CE-SSFP was validated against manual delineation in multi-center, multi-vendor studies with low bias and high regional agreement. Bias and variability was similar to inter-observer variability of manual delineation and inter-observer variability was decreased by automatic segmentation. Thus, the proposed automatic segmentation can be used to reduce subjectivity in quantification of MaR in RCT.

Clinical trial registration: NCT01379261 .

NCT01374321 .

Files

12880_2016_124_MOESM1_ESM.pdf

Files (2.7 MB)

Name Size Download all
md5:4f3f11db7ae7734c06f60af5199ecd93
58.4 kB Preview Download
md5:ab73ce67606da11564acf0f892890da8
2.6 MB Preview Download
md5:2795ed31d9088e5e517c865d8fc7c1d8
27.2 kB Download

Additional details

Funding

MITOCARE – Treatment of reperfusion injury using a mitochondrial targeted approach: towards a better understanding of the disease. 261034
European Commission