{"Pixels":{"Name":"New Pixels","Schema_ID":"Pixels.json","ID":"e837e113-9c48-4e3a-b136-8b19d3c623b6","Tier":3,"ModelVersion":"2.01.1","Extension":"Core","Domain":"ImageAcquisitionSettings","Category":"Image","SizeC":1,"Interleaved":false,"PhysicalSizeX":0.27675527915059667,"PhysicalSizeY":0.27675527915059667,"BigEndian":false,"PhysicalSizeZ":1.0899999999999999,"SignificantBits":16,"DimensionOrder":"XYCZT","SizeT":1,"SizeX":187,"SizeZ":57,"SizeY":138,"PixelType":"uint16","PhysicalSizeXUnit":"µm","PhysicalSizeYUnit":"µm","PhysicalSizeZUnit":"nm","TimeIncrementUnit":"s"},"Name":"01_MARCELLO_PDMS_nilered_4nm_Subset_nr1_AS","Schema_ID":"Image.json","ID":"56dd5c80-fa55-466d-ab2c-c68af90d4e27","Tier":3,"ValidationTier":1,"ModelVersion":"2.01.1","AppVersion":"1.6.19-b1","InstrumentName":"01_MARCELLO_ULiverpool_CCI_Zeiss_AxioObserZ1_LSM710","InstrumentID":"56e36431-0dff-4346-9d25-a97859db99f7","Extension":"Core","Domain":"ImageAcquisitionSettings","Category":"Image","AcquisitionSoftware":["AcquisitionSoftware/df3663ce-f549-4f70-ab65-c44e1ff938ed"],"MicroscopeStandSettings":{"Name":"MicroscopeStandSettings","ID":"7dad4c4c-cfae-441e-b726-7f4bf414c67e","Tier":2,"Schema_ID":"MicroscopeStandSettings.json","ModelVersion":"2.01.1","Extension":"Basic","Domain":"ImageAcquisitionSettings","Category":"Settings"},"ObjectiveSettings":{"ImmersionLiquid":{"Type":"Water","RefractiveIndex":1.333,"Name":"ImmersionLiquid","ID":"07ce15c1-56da-4aa8-96ab-5b13613fcf8d","Tier":1,"Schema_ID":"ImmersionLiquid.json","ModelVersion":"2.01.1","Extension":"Basic","Domain":"Experimental","Category":"SamplePreparation","Manufacturer":"no immersion liquid","Model":"NA","CatalogNumber":"NA"},"Name":"ObjectiveSettings","ID":"f6128b35-1456-48c4-a9e1-b963536d8372","Component_ID":"25c84b1e-4abc-4720-a7e0-3e3341beefcb","Tier":1,"Schema_ID":"ObjectiveSettings.json","ModelVersion":"2.01.1","Extension":"Core","Domain":"ImageAcquisitionSettings","Category":"Settings","TemperatureUnit":"°C"},"Channels":[{"Name":"Ch2 - 561 (Nile Red)","ID":"08348b0e-1759-40ad-b351-fc8560cc62b0","Tier":1,"Schema_ID":"Channel.json","ModelVersion":"2.01.1","Extension":"Core","Domain":"ImageAcquisitionSettings","Category":"Image","Fluorophore":{"Name":"Nile Red","ID":"1636eff9-eabc-4640-ba67-ff198de499d5","Tier":1,"Schema_ID":"Fluorophore.json","ModelVersion":"2.01.1","Extension":"Basic","Domain":"Experimental","Category":"SamplePreparation","EmissionWavelength":652.1300000000001,"ExcitationWavelength":558,"Manufacturer":"Sigma-Aldrich","Model":"Nile Red for Microscopy","CatalogNumber":"72485","SpecsFile":"https://www.sigmaaldrich.com/catalog/product/sigma/72485?lang=en®ion=US","ExcitationWavelengthUnit":"nm","EmissionWavelengthUnit":"nm","Type":"Other","PrimaryPublicationID":"Nile red: a selective fluorescent stain for intracellular lipid droplets. Greenspan P, et al. The Journal of Cell Biology 100(3), 965-973, (1985); DOI: 10.1083/jcb.100.3.965"},"SamplesPerPixel":1,"LightPath":{"Name":"LightPath 0","ID":"ff8d03df-f0ee-4a88-bf4c-16115b3ff908","Tier":1,"Schema_ID":"LightPath.json","ModelVersion":"2.01.1","Extension":"Core","Domain":"ImageAcquisitionSettings","Category":"LightPath","ComponentSettings":{"Detector":{"Name":"PointDetectorSettings","ID":"40f778ed-ee85-44cb-bb18-453fec8107eb","Component_ID":"3bcefc51-6301-4139-97ac-a30b53ff089f","Tier":1,"Schema_ID":"PointDetectorSettings.json","ModelVersion":"2.01.1","Extension":"Basic","Domain":"ImageAcquisitionSettings","Category":"Settings","EffectiveBitDepth":"12bit","AmplificationGain":1,"Offset":0,"AnalogGain":467,"VoltageUnit":"mV","Voltage":-1},"AdditionalSlot_2":[],"Dichroic":{"Name":"MirroringDeviceSettings","ID":"fcca58b2-c3fd-4c94-9e23-0388b672a0da","Component_ID":"c049ae59-b272-43dc-ae7f-9b15c65d7049","Tier":2,"Schema_ID":"MirroringDeviceSettings.json","ModelVersion":"2.01.1","Extension":"Basic","Domain":"ImageAcquisitionSettings","Category":"Settings"},"EmissionFilter":{"Name":"EmissionFilter","ID":"e9c7681f-3bda-4f21-ba65-b7863a838925","Component_ID":"a1c552bf-12b3-45a6-b4b7-f2ecf29182f5"},"AdditionalSlot_1":[],"AdditionalSlot_7":[],"AdditionalSlot_3":[],"AdditionalSlot_8":[],"LightSource":{"Name":"LightSourceSettings","ID":"137d33af-71c3-49cb-a352-7152590d869d","Component_ID":"9ae10d64-8ece-42be-8676-5ab8e74cdefa","Tier":1,"Schema_ID":"LightSourceSettings.json","ModelVersion":"2.01.1","Extension":"Core","Domain":"ImageAcquisitionSettings","Category":"Settings","lluminationPowerSettingsUnit":"µW","IlluminationPowerSettings":560,"UsedAcoustoOpticalDevice":true}}},"Color":-16776961,"IlluminationType":"Confocal_Fluorescence_array-raster-scan","ImagingMethod":"LaserScanningConfocalMicroscopy","ContrastMethod":"Fluorescence","Description":"For this Channel samples were stained with Nile Red. Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets[1] and proteins.Nile Red detects lysosomes and lysosome-related organelles."}],"SamplePositioningSettings":[{"Name":"SamplePositioningSettings","ID":"7236c49a-9c84-440c-8d03-222150789e46","Component_ID":"37236578-de72-4cda-8cae-c3d8b46535c3","Tier":1,"Schema_ID":"SamplePositioningSettings.json","ModelVersion":"2.01.1","Extension":"Basic","Domain":"ImageAcquisitionSettings","Category":"Settings"}],"Description":"These are the acquisition settings utilized for the acquisition of the PDMS_nilered_4nm_Subset #1 image using the Carl Zeiss Microscopy Axio Observer Z1 (with LSM 710 scanhead) microscope of the Centre for Cell Imaging (CCI) of the University of Liverpool (core director Marco Marcello)."}