Published January 12, 2022
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Human kidney cortex spectral confocal reference dataset 1
- 1. University of Nebraska Medical Center
- 2. Indiana University of School of Medicine
Description
Confocal image volume of human kidney cortex stained with antibodies for Uromodulin, AQP1, MPO, CD68, CD3 and Siglec-8 and DAPI and phalloidin. Tissue section was mounted in Prolong Gold and imaged at 20X NA 0.75 on an Leica SP8 with spectral imaging and unmixed using LASX software. Protocol at: 10.17504/protocols.io.9avh2.
Channels, in order: DAPI, Phalloidin (F-Actin), Uromodulin, AQP1, MPO, CD68, CD3 and Siglec-8
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Kidney_Cortex_Human_Spectral_1.tif
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Additional details
Related works
- Cites
- 10.17504/protocols.io.9avh2 (DOI)
- Is described by
- Preprint: 10.1101/2021 (DOI)
Funding
- Center for Advanced Renal Microscopic Analysis 3P50DK061594-04S1
- National Institutes of Health