In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture
Creators
- 1. King Abdullah University of Science and Technology
Description
Mammalian cell cultures are a keystone resource in biomedical research, but the results of published experiments often suffer from reproducibility challenges. This has led to a focus on the influence of cell culture conditions on cellular responses and reproducibility of experimental findings. Here, we perform frequent in situ monitoring of dissolved O2 and CO2 with optical sensor spots and contemporaneous evaluation of cell proliferation and medium pH in standard batch cultures of three widely used human somatic and pluripotent stem cell lines. We collate data from the literature to demonstrate that standard cell cultures consistently exhibit environmental instability, indicating that this may be a pervasive issue affecting experimental findings. Our results show that in vitro cell cultures consistently undergo large departures of environmental parameters during standard batch culture. These findings should catalyze further efforts to increase the relevance of experimental results to the in vivo physiology and enhance reproducibility.
Notes
Environmental parameter data of in situ measurements in the medium of 12 different cell lines. Missing values represent unavailable data. For columns that contain quantitative data (e.g. working volume = 40mL, time of measurements = 24 hours), the "unit" column to the right contains the unit of measurement. Publications reported CO2 and O2 concentrations in different units (mmHg, % air saturation, and absolute %) and were converted into units of an absolute percent (%) under the assumption that experiments were conducted at ambient atmospheric pressure (101.325 kPa). Columns labeled "units reported" represent the values reported in the original publication that were converted into units of an absolute %.
Funding provided by: King Abdullah University of Science and Technology
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004052
Award Number: Baseline funding to CMD
Funding provided by: King Abdullah University of Science and Technology
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004052
Award Number: URF/1/3412-01-01
Funding provided by: King Abdullah University of Science and Technology
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004052
Award Number: BAS/1/1080-01
Files
Klein_et_al._dataset.csv
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