Synthetic cryo electron subtomograms containing biomolecular complexes with continuous conformational variability, used for validating TomoFlow method

Two datasets used for validating TomoFlow method, an optical-flow based approach for analyzing continuous conformational variability of biomolecular complexes in cryo electron subtomograms. The TomoFlow method and the methods used to synthesize the two test datasets have been fully described in the following article: "M. Harastani, M. Eltsov, A. Leforestier, S. Jonic, TomoFlow: Analysis of continuous conformational variability of macromolecules in cryogenic subtomograms based on 3D dense optical flow, Journal of Molecular Biology (2021), doi: https://doi.org/10.1016/j.jmb.2021.167381". Additionally, this article describes a test of TomoFlow using one experimental cryo electron tomography dataset (available in EMPIAR and EMDB databases under the accession codes EMPIAR-10679 and EMD-12699).