Modules > Objects > Measure > Intensity
Measure intensity along path
Measures the intensity profile along the pixel-wide backbone of an object and outputs this profile to .
Description
Measures the intensity profile along the pixel-wide backbone of an object and outputs this profile to .xlsx file. Input objects are skeletonised to single pixel-wide representations prior to measurement; however, pre-skeletonised objects can also be processed.
Output results are stored in a multi-sheet .xlsx file, where each sheet includes the profile for a specific input image. Each row of a sheet contains the profile for a single object. Profiles are linearly-interpolated such that each measured position along a profile is 1px from the previous.
Note: Objects must either form a single line (i.e. not contain multiple branches) or reduce to a single line during skeletonisation. No profile will be recorded for any objects which fail this requirement.
Parameters
Parameter | Description |
Input objects | Objects for which intensity profiles will be generated. |
Measure another image | Include another image from the workspace to be measured. Each separate image will be measured at the same spatial points and be saved to a separate sheet of the .xlsx file. |
Input image | Image for which the intensity profile will be measured. Results from this profile will be added to a separate sheet in the .xlsx file. |
Include timepoints | Include a column recording the timepoint that the objects were present in. |
Save name mode | Controls how saved profile file names will be generated.
|
File name | Filename for saved distance file. Note: Care should be taken with this when working in batch mode as it's easy to continuously write over output files from other runs. |
Append series mode | Controls if any series information should be appended to the end of the filename. This is useful when working with multi-series files, as it should help prevent writing files from multiple runs with the same filename. Series numbers are prepended by "S". Choices are: None, Series name, Series number. |
Append date/time mode | Controls under what conditions the time and date will be appended on to the end of the profile file filename. This can be used to prevent accidental over-writing of files from previous runs:
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Add filename suffix | A custom suffix to be added to each filename. |