Figs. 1–2
Material examined: 25 specimens and 15 eggs, one of them with fully developed embryo. Two specimens were mounted in polyvinyl lactophenol. All the other specimens and the eggs were mounted in Hoyer’s medium. One specimen was in simplex stage.
Type repository: The holotype (slide N. 5295), four paratypes and two eggs are deposited in the collection of Binda and Pilato (Museo del Dipartimento di Biologia Animale “Marcello La Greca”, Università di Catania); the other paratypes and eggs (slides CII- 28 to CII- 57) in the collection of P. Fontoura (Department of Zoology and Anthropology, Faculty of Sciences, University of Porto).
Specific diagnosis: Cuticle with variably shaped pores arranged in 9 wide transverse bands, the cephalic and caudal bands widest; granulation on legs absent. Eye spots present. Buccal tube narrow; buccal armature with peribuccal papulae as in all species of Minibiotus; bands of teeth seem to be absent but this feature needs to be confirmed; two very fine latero-ventral transverse ridges and a single transverse dorsal ridge present; medioventral ridge not visible. Pharyngeal bulb with apophyses, three small macroplacoids and microplacoid. Robust claws of hufelandi type with well developed accessory points and smooth lunules. Eggs, freely laid, with long conical processes, with bifurcate apices and terminal filaments often broken; process surface and egg shell with widely spaced granulation.
Description of the holotype: Body length 369 µm, colorless; large eye spots consisting of a few pigment granules. Cuticle with small pores (longer diameter of elliptical pores up to about 2.0 µm long) arranged in 9 wide transverse bands, cephalic and caudal of which are widest; pores variable in shape: roundish, oval and, very few, trilobed (Fig. 1 A). Ventral surface of body with few roundish pores. Pores also present on legs (Fig. 1 A) where no granulation is present.
Mouth antero-ventral; buccal cavity very small (Fig. 1 B, C); buccal armature without peribuccal lamellae and with peribuccal papulae as in all species of Minibiotus; bands of teeth seem to be absent but this feature needs to be confirmed; two very fine latero-ventral transverse ridges and a single transverse dorsal ridge present; a medioventral ridge not visible.
Rigid buccal tube narrow, 32.7 µm long and 3.0 µm wide externally (pt = 9.2), with an unmarked anterior bend (Fig. 1 C). Slight thickening of buccal tube wall present caudal to stylet supports (Fig. 1 B). Ventral lamina not very long (pt = 55.7 measured in lateral view). Stout stylet supports inserted on buccal tube at 67.3 % of its length (pt = 67.3). Pharyngeal bulb with large triangular apophyses and three small macroplacoids and microplacoid (Fig. 1 B, C); first macroplacoid, pear shaped, partially lying under apophysis as in other species of the genus, 4.5 µm long (pt = 13.8); second macroplacoid, roundish, 3.6 µm (pt = 11.0); third macroplacoid 3.9 µm long (pt = 11.9). Microplacoid 1.7 µm long (pt = 5.2). Macroplacoid row length, 12.6 µm long (pt = 38.5); entire row of placoids 13.9 µm long (pt = 42.5).
Robust claws of hufelandi type, with well-developed accessory points on main branches (Fig. 1 D, E) particularly prominent on hind legs. External and internal claws of legs II and III 10.5 µm long (pt = 32.1) and 10.0 µm long (pt = 30.6) respectively, including accessory points and peduncle. Posterior and anterior claws of fourth pair of legs 13.0 µm long (pt = 39.8) and 12.0 µm long (pt = 36.7) respectively.
Smooth lunules, better developed on fourth pair of legs (about 3.0 µm longer diameter), present. Two small, faint cuticular bars, difficult to see, present below lunules on first three pairs of legs.
The measurements of some structures of holotype and four paratypes are shown in Table 1.
Minibiotus xavieri sp. nov.
Eggs: Eggs, freely laid, spherical or slightly oval, with dotted shell and long conical processes with bifurcate apices and two or more terminal filaments (often broken) (Fig. 2, black arrows); some processes forked in a more proximal position (Fig. 2 A–C white arrows). Diameter 56.0–79.0 µm excluding processes, 80 and 99.2 µm, including these structures. Processes, 20–23 around circumference, 75–95 in hemisphere, 10.6 –19.0 µm high and basal diameter of 3.7–6.6 µm. Egg processes 3.3–5.8 µm apart. Egg process surface and egg shell with obvious, well-spaced granulation (Fig. 2 C, D).
*) Unfortunately, due to a qui pro quo, Binda and Pilato, in the paper of 1992, wrote that the pt value relative to the buccal tube in M. furcatus is 7.94.
The paratypes are similar to the holotype in both qualitative and metric characters. The smallest measured specimen is 275 µm long (structures not measured) and the largest specimen 410 µm.
Etymology: The new species is dedicated in honor of Alberto Xavier da Cunha, pioneer in the study of Portuguese tardigrades.
Differential diagnosis: We compared Minibiotus xavieri sp. nov. with the species of the genus having cuticular pores of variable shape (also trilobate) arranged in more or less defined transverse bands, buccal tube with an anterior bend and no posterior bend, and pharyngeal bulb with three macroplacoids and microplacoid. These characters considered, the most similar species are: Minibiotus eichorni Michalczyk & Kaczmarek, 2004, M. orthofasciatus, M. vinciguerrae, and M. furcatus; the new species differs from them in some qualitative and quantitative characters of both animals and eggs.
The eggs of Minibiotus eichorni are not known but the specimens of Minibiotus xavieri sp. nov. differ from M. eichorni in the distribution of the cuticular pores (9 transverse bands instead of 8); in having smaller cuticular pores (longer diameter of elliptical pores up to 2 µm long in the holotype of Minibiotus xavieri sp. nov. 369 µm long, up to 3.5 µm in specimens of M. eichorni 318 µm long); in lacking pores with 4 or 5 arms; in lacking granulation on the legs and in having claws with more prominent accessory points.
The new species differs from Minibiotus orthofasciatus in the distribution and size of the cuticular pores: Minibiotus xavieri sp. nov. has a lower number of transverse bands (9 instead of 11) each with a higher number of pores; the diameter of pores is the same but in specimens of very different body length; in having longer microplacoid (Table 2); in having slightly longer claws (Table 2 and Figs. 1 D, E and 3 A) and in producing quite different eggs (Figs. 2 and 3 B, C).
Minibiotus xavieri sp. nov. differs from Minibiotus vinciguerrae in having shorter body length, in lacking granulation on the legs; in having the cuticular pores (never quadrilobate) clearly arranged in transverse bands; in having less slender claws (Figs. 1 D, E and 3 E, F), and in characters of the eggs (the processes are less numerous, 20–23 in the circumference, 75–95 in the hemisphere in M. xavieri sp. nov., 26 and 110 respectively in M. vinciguerrae; they are longer, 10.6–19 µm in the new species, 8–9 µm in M. vinciguerrae; the process surface and the egg shell are clearly dotted while they are smooth in M. vinciguerrae) (Figs. 2 and 3 D).
The new species differs from Minibiotus furcatus in having few trilobate cuticular pores and no quadrilobate ones; narrower buccal tube (Table 2), and very different eggs (the egg processes are up to 19 µm long, with a well-spaced granulation and bifurcate apex in M. xavieri sp. nov., while in M. furcatus they are up to 6 µm long, smooth and never bifurcate; in addition the egg shell is clearly dotted in M. xavieri sp. nov. and smooth in M. furcatus).
As stressed by Pilato & Lisi (2006), by Guidetti et al. (2007) and by Fontoura et al. (2009), some species described as belonging to the genus Macrobiotus should be transferred to the genus Minibiotus. For this reason we think that it is appropriate to compare Minibiotus xavieri sp. nov. with three species of Macrobiotus (M. pustulaus Ramazzotti, 1959, M. lazzaroi and M. pseudofurcatus) which are similar to the new species, and whose systematic position needs to be analyzed because the examination of the type material did not confirm the presence of peribuccal lamellae (a generic character of Macrobiotus).
Minibiotus xavieri sp. nov. differs from Macrobiotus pustulatus Ramazzott i, 1959 in having eye spots, clearly smaller cuticular pores (diameter of largest pores in M. xavieri sp. nov. about 2 µm, in M. pustulatus up 6-7 µm), longer placoid row, and different eggs.
Minibiotus xavieri sp. nov. differs from Macrobitous lazzaroi in having slightly smaller and more numerous cuticular pores (Figs. 1 A and 4 A), narrower buccal tube (Table 2), and quite different eggs.
Minibiotus xavieri sp. nov. differs from Macrobiotus pseudofurcatus in having narrower buccal tube (Table 2), claws shorter (Table 2) and different in shape (Figs. 1 D, E and 4 B, C), (the difference in length between main and secondary branches is lower than in M. pseudofurcatus), and in some characters of the eggs: they are slightly smaller (diameter 56–79 µm without processes in the new species, 83–90 µm in M. pseudofurcatus); the processes are less numerous (20–23 in the circumference and 75–95 in the hemisphere in Minibiotus xavieri, 30–31 and about 130 respectively in M. pseudofurcatus); the process surface has a wellspaced granulation while it is smooth in M. pseudofurcatus (Figs. 2 C, D and 4 D); the proximal portion of the processes, below the bifurcation, is generally longer than in the eggs of Macrobiotus pseudofurcatus (Figs. 2 and 4 D); the egg shell has a more evident granulation in M. xavieri.
Maucci & Durante-Pasa (1984 a) reported M. pseudofurcatus from Portugal. Keeping in mind the similarities between Minibiotus xavieri sp. nov. and Macrobiotus pseudofurcatus, we examined one specimen from Portugal attributed by those authors to M. pseudofurcatus. We can exclude the proposition that the Portuguese specimens recorded by Maucci belong to this species and state that they very probably belong to Minibiotus xavieri sp. nov.
Macrobiotidae Thulin, 1928
Calcarobiotus (Calcarobiotus) sp. Dastych, 1993 Macrobiotus cf. harmsworthi Murray, 1907 Macrobiotus hufelandi Schultze, 1834
Macrobiotus lusitanicus Maucci & Durante Pasa, 1984 b Macrobiotus recens Cuénot, 1932
Macrobiotus cf. richtersi Murray, 1911
Minibiotus cf. intermedius (Plate, 1889)
Minibiotus orthofasciatus Fontoura, Pilato, Lisi & Morais, 2009 Minibiotus xavieri sp. nov.
Eohypsibiidae Bertolani & Kristensen, 1987 (nomen novum for Amphibolidae Bertolani, 1981) Bertolanius weglarskae (Dastych, 1972)
Hypsibiidae Pilato, 1969
Hypsibius seychellensis Pilato, Binda & Lisi, 2006 Isohypsibius josephi (Iharos, 1964)
Isohypsibius prosostomus (Thulin, 1928)
Isohypsibius sattleri (Richters, 1902)
Diphascon (Diphascon) patanei Binda & Pilato, 1971 Diphascon (Diphascon) pingue (Marcus, 1936 ) Astatumen trinacriae (Arcidiacono, 1962)
Milnesiidae Ramazzotti, 1962
Milnesium tardigradum Doyère, 1840