Description

Measures the intensity profile along the pixel-wide backbone of an object and outputs this profile to .xlsx file. Input objects are skeletonised to single pixel-wide representations prior to measurement; however, pre-skeletonised objects can also be processed.

Output results are stored in a multi-sheet .xlsx file, where each sheet includes the profile for a specific input image. Each row of a sheet contains the profile for a single object. Profiles are linearly-interpolated such that each measured position along a profile is 1px from the previous.

Note: Objects must either form a single line (i.e. not contain multiple branches) or reduce to a single line during skeletonisation. No profile will be recorded for any objects which fail this requirement.

Parameters

Parameter Description
Input objectsObjects for which intensity profiles will be generated.
Measure another imageInclude another image from the workspace to be measured. Each separate image will be measured at the same spatial points and be saved to a separate sheet of the .xlsx file.
Input imageImage for which the intensity profile will be measured. Results from this profile will be added to a separate sheet in the .xlsx file.
Include timepointsInclude a column recording the timepoint that the objects were present in.
Save name modeControls how saved profile file names will be generated.
  • "Match input file name" Use the same name as the root file for this workspace (i.e. the input file in "Input control".
  • "Specific name" Use a specific name for the output file. Care should be taken with this when working in batch mode as it's easy to continuously write over output files from other runs.
File nameFilename for saved distance file. Note: Care should be taken with this when working in batch mode as it's easy to continuously write over output files from other runs.
Append series modeControls if any series information should be appended to the end of the filename. This is useful when working with multi-series files, as it should help prevent writing files from multiple runs with the same filename. Series numbers are prepended by "S". Choices are: None, Series name, Series number.
Append date/time modeControls under what conditions the time and date will be appended on to the end of the profile file filename. This can be used to prevent accidental over-writing of files from previous runs:
  • "Always" Always append the time and date on to the end of the filename.
  • "If file exists" Only append the time and date if the results file already exists.
  • "Never" Never append time and date (unless the file is open and unwritable).
Add filename suffixA custom suffix to be added to each filename.