Culturing Patient-Derived Malignant Hematopoietic Stem Cells in Engineered and Fully Humanized 3D Niches

Data underlying the figures in the publication “Culturing patient-derived malignant hematopoietic stem cells in engineered and fully humanized 3D niches”, published in PNAS, 2021, 118 (40) e2114227118.

DOI: 10.1073/pnas.2114227118

Table of contents:

1. AML data_Gene expression: qPCR data related to AML samples.

2. AML data_Protein: Luminex data related to AML samples.

3. AML data_Supernatant vs Niche: FACS data comparing AML cells in the supernatant and niche compartments.

4. AML data_Supernatant: FACS data showing AML cells in the supernatant along the time.

5. Chemotherapy_2D_FACS: FACS data showing leukemic cell viability in 2D.

6. Chemotherapy_3D_FACS: FACS data showing leukemic cell viability in 3D (engineered niches).

7. MPN data: FACS data related to MPN samples.

8. SV-N_FACS: FACS data characterizing SV-N.

9. SV-N_Gene expression: qPCR data characterizing SV-N.

10. UCSD-AML1_Niche_FACS: FACS data showing UCSD-AML1 cells in the niche at final analysis.

11. UCSD-AML1_Niche_Gene expression: qPCR data showing UCSD-AML1 cells in the niche at final analysis.

12. UCSD-AML1_Supernatant_FACS: FACS data showing UCSD-AML1 cells in the supernatant along the time.

13. UCSD-AML1_Supernatant_Gene expression: qPCR data related to UCSD-AML1 cells in the supernatant along the time.

14. UCSD-AML1_Supernatant_Protein: Luminex data related to UCSD-AML1 cells in the supernatant along the time.

15. Images engineered niches: Zip archive containing images of engineered niches.