This AMAKIHI_CORT_EXPERIMENT_readme.txt file was generated on 2021-10-5 by GABRIELLE R. NAMES GENERAL INFORMATION 1. Title of Dataset: Dataset for Stress in paradise: effects of elevated corticosterone on immunity and avian malaria resilience in a Hawaiian passerine 2. Author Information Principal Investigator Contact Information Name: Gabrielle Names Institution: University of California Davis Email: grnames@ucdavis.edu 3. Date of data collection: 2019 4. Geographic location of data collection: Hawaii Island, Hawaii, USA SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: None 2. Links to publications that cite or use the data: https://doi.org/10.1242/jeb.242951 DATA & FILE OVERVIEW 1. File List: AMAKIHI_CORT_EXPERIMENT_CORT.csv AMAKIHI_CORT_EXPERIMENT_HLHA.csv AMAKIHI_CORT_EXPERIMENT_LEUKOCYTES.csv AMAKIHI_CORT_EXPERIMENT_HEMATOCRIT.csv AMAKIHI_CORT_EXPERIMENT_PLASMODIUM.csv 2. Are there multiple versions of the dataset? No METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data can be found in the Materials and Methods section of the article. DATA-SPECIFIC INFORMATION FOR: AMAKIHI_CORT_EXPERIMENT_CORT.csv 1. Number of variables: 13 2. Number of cases/rows: 120 3. Variable List: Bird_id: each individual bird received a unique number identifier (1 to 40) Sample_day: the day the sample was collected Sample_month: the month the sample was collected Sample_year: the year the sample was collected Experiment_day: the day of the experiment period the sample was collected; 0 = start of the experiment, 2 = two days after start of the experiment; 4 = four days after start of the experiment Experiment_round: the round of the experiment in which the bird was included; the experiment was run in 8 total rounds (1-8) Corticosterone_value_ng_per_mL: the circulating corticosterone level of the bird in ng mL-1 obtained via radioimmunoassay Aviary_room: the aviary room in which the bird was housed during the acclimation and experiment periods; A = aviary room A, B = aviary room B Implant_type: the implant type the bird received during the experiment; CORT = experimental implants filled with crystalline corticosterone, sham = control implants that were left empty Elevation: the elevation at which the individual was captured; high = high elevation (> 1500 m above sea level, asl), low = low elevation (< 700 m asl) Side_of_island: the side of the island at which the individual was captured; dry = dry side of the island (leeward), wet = wet side of the island (windward) Site: the site name (see corresponding article for additional details about field sites) at which the individual was captured Malaria_infection_status: the individual avian malaria infection status determined using nested Polymerase Chain Reaction (see corresponding article for additional details about the methods); positive = infected, negative = uninfected 4. Missing data codes: NA DATA-SPECIFIC INFORMATION FOR: AMAKIHI_CORT_EXPERIMENT_HLHA.csv 1. Number of variables: 14 2. Number of cases/rows: 110 3. Variable List: Bird_id: each individual bird received a unique number identifier (1 to 40) Sample_day: the day the sample was collected Sample_month: the month the sample was collected Sample_year: the year the sample was collected Experiment_day: the day of the experiment period the sample was collected; 0 = start of the experiment, 2 = two days after start of the experiment; 4 = four days after start of the experiment Experiment_round: the round of the experiment in which the bird was included; the experiment was run in 8 total rounds (1-8) Agglutination_score: the agglutination score of the bird obtained via the hemolysis-hemagglutination assay; score was calculated as log2(W + 1) where W was the last dilution well in which agglutination was detected Aviary_room: the aviary room in which the bird was housed during the acclimation and experiment periods; A = aviary room A, B = aviary room B Implant_type: the implant type the bird received during the experiment; CORT = experimental implants filled with crystalline corticosterone, sham = control implants that were left empty Elevation: the elevation at which the individual was captured; high = high elevation (> 1500 m above sea level, asl), low = low elevation (< 700 m asl) Side_of_island: the side of the island at which the individual was captured; dry = dry side of the island (leeward), wet = wet side of the island (windward) Site: the site name (see corresponding article for additional details about field sites) at which the individual was captured Malaria_infection_status: the individual avian malaria infection status determined using nested Polymerase Chain Reaction (see corresponding article for additional details about the methods); positive = infected, negative = uninfected Plasma_volume_ng_per_mL: the plasma volume per well at which the hemolysis-hemagglutination assay was run in ng mL-1 ; samples were run at 2.5 ng mL-1 per well or 5 ng mL-1 per well 4. Missing data codes: NA DATA-SPECIFIC INFORMATION FOR: AMAKIHI_CORT_EXPERIMENT_LEUKOCYTES.csv 1. Number of variables: 22 2. Number of cases/rows: 95 3. Variable List: Bird_id: each individual bird received a unique number identifier (1 to 40) Sample_day: the day the sample was collected Sample_month: the month the sample was collected Sample_year: the year the sample was collected Experiment_day: the day of the experiment period the sample was collected; 0 = start of the experiment, 2 = two days after start of the experiment; 4 = four days after start of the experiment Experiment_round: the round of the experiment in which the bird was included; the experiment was run in 8 total rounds (1-8) Number_fields: the number of slide scoring fields (representing approximately 100 erythrocytes each) per sample Total_lymphocytes: the total number of lymphocytes across all scoring fields Total_heterophils: the total number of heterophils across all scoring fields Total_monocytes: the total number of monocytes across all scoring fields Total_eosinophils: the total number of eosinophils across all scoring fields Total_basophils: the total number of basophils across all scoring fields Total_leukocytes: the total number of leukocytes across all scoring fields Leukocytes_per_field: the number of leukocytes per scoring field Leukocytes_per_erythrocyte: the number of leukocytes per approximate erythrocyte count Aviary_room: the aviary room in which the bird was housed during the acclimation and experiment periods; A = aviary room A, B = aviary room B Implant_type: the implant type the bird received during the experiment; CORT = experimental implants filled with crystalline corticosterone, sham = control implants that were left empty Elevation: the elevation at which the individual was captured; high = high elevation (> 1500 m above sea level, asl), low = low elevation (< 700 m asl) Side_of_island: the side of the island at which the individual was captured; dry = dry side of the island (leeward), wet = wet side of the island (windward) Site: the site name (see corresponding article for additional details about field sites) at which the individual was captured Malaria_infection_status: the individual avian malaria infection status determined using nested Polymerase Chain Reaction (see corresponding article for additional details about the methods); positive = infected, negative = uninfected Hematocrit: the percent hematocrit (packed cell volume) measured from centrifuged capillary tubes 4. Missing data codes: NA DATA-SPECIFIC INFORMATION FOR: AMAKIHI_CORT_EXPERIMENT_HEMATOCRIT.csv 1. Number of variables: 13 2. Number of cases/rows: 118 3. Variable List: Bird_id: each individual bird received a unique number identifier (1 to 40) Sample_day: the day the sample was collected Sample_month: the month the sample was collected Sample_year: the year the sample was collected Experiment_day: the day of the experiment period the sample was collected; 0 = start of the experiment, 2 = two days after start of the experiment; 4 = four days after start of the experiment Experiment_round: the round of the experiment in which the bird was included; the experiment was run in 8 total rounds (1-8) Hematocrit: the percent hematocrit (packed cell volume) measured from centrifuged capillary tubes Aviary_room: the aviary room in which the bird was housed during the acclimation and experiment periods; A = aviary room A, B = aviary room B Implant_type: the implant type the bird received during the experiment; CORT = experimental implants filled with crystalline corticosterone, sham = control implants that were left empty Elevation: the elevation at which the individual was captured; high = high elevation (> 1500 m above sea level, asl), low = low elevation (< 700 m asl) Side_of_island: the side of the island at which the individual was captured; dry = dry side of the island (leeward), wet = wet side of the island (windward) Site: the site name (see corresponding article for additional details about field sites) at which the individual was captured Malaria_infection_status: the individual avian malaria infection status determined using nested Polymerase Chain Reaction (see corresponding article for additional details about the methods); positive = infected, negative = uninfected 4. Missing data codes: NA DATA-SPECIFIC INFORMATION FOR: AMAKIHI_CORT_EXPERIMENT_PLASMODIUM.csv 1. Number of variables: 17 2. Number of cases/rows: 19 3. Variable List: Bird_id: each individual bird received a unique number identifier (1 to 40) Sample_day: the day the sample was collected Sample_month: the month the sample was collected Sample_year: the year the sample was collected Experiment_day: the day of the experiment period the sample was collected; 0 = start of the experiment, 2 = two days after start of the experiment; 4 = four days after start of the experiment Experiment_round: the round of the experiment in which the bird was included; the experiment was run in 8 total rounds (1-8) Number_fields: the number of slide scoring fields (representing approximately 100 erythrocytes each) per sample Total_plasmodium: the total number of Plasmodium parasites across all scoring fields Plasmodium_per_field: the number of leukocytes per scoring field Plasmodium_per_erythrocyte: the number of leukocytes per approximate erythrocyte count Aviary_room: the aviary room in which the bird was housed during the acclimation and experiment periods; A = aviary room A, B = aviary room B Implant_type: the implant type the bird received during the experiment; CORT = experimental implants filled with crystalline corticosterone, sham = control implants that were left empty Elevation: the elevation at which the individual was captured; high = high elevation (> 1500 m above sea level, asl), low = low elevation (< 700 m asl) Side_of_island: the side of the island at which the individual was captured; dry = dry side of the island (leeward), wet = wet side of the island (windward) Site: the site name (see corresponding article for additional details about field sites) at which the individual was captured Malaria_infection_status: the individual avian malaria infection status determined using nested Polymerase Chain Reaction (see corresponding article for additional details about the methods); positive = infected, negative = uninfected Hematocrit: the percent hematocrit (packed cell volume) measured from centrifuged capillary tubes 4. Missing data codes: NA