Parameters

Input image: Image from the workspace to apply unmixing to. This can be stored as either an RGB or composite image.

Output image 1: When selected, the first stain in the stain matrix will be output to the workspace with the name specified by "Output image 1 name"

Output image 1 name: Name to assign to first stain image, if output to the workspace.

Output image 2: When selected, the second stain in the stain matrix will be output to the workspace with the name specified by "Output image 2 name"

Output image 2 name: Name to assign to second stain image, if output to the workspace.

Output image 3: When selected, the third stain in the stain matrix will be output to the workspace with the name specified by "Output image 3 name"

Output image 3 name: Name to assign to third stain image, if output to the workspace.

Stain model: Stain models to apply to input image. If set to "Custom (user values)" the individual RGB components for each channel can be specified. Model choices are: Alcian blue & H,Azan-Mallory,Brilliant_Blue,CMY,FastRed FastBlue DAB,Feulgen Light Green,Giemsa,H AEC,H&E,H&E 2,H&E DAB,H DAB,H PAS,Masson Trichrome,Methyl Green DAB,RGB,Custom (user values)

Stain 1 (red): Red component of stain 1. Value specified in range 0-1.

Stain 1 (green): Green component of stain 1. Value specified in range 0-1.

Stain 1 (blue): Blue component of stain 1. Value specified in range 0-1.

Stain 2 (red): Red component of stain 2. Value specified in range 0-1.

Stain 2 (green): Green component of stain 2. Value specified in range 0-1.

Stain 2 (blue): Blue component of stain 2. Value specified in range 0-1.

Stain 3 (red): Red component of stain 3. Value specified in range 0-1.

Stain 3 (green): Green component of stain 3. Value specified in range 0-1.

Stain 3 (blue): Blue component of stain 3. Value specified in range 0-1.