Description

Measures the intensity profile along the pixel-wide backbone of an object and outputs this profile to .xlsx file. Input objects are skeletonised to single pixel-wide representations prior to measurement; however, pre-skeletonised objects can also be processed.

Output results are stored in a multi-sheet .xlsx file, where each sheet includes the profile for a specific input image. Each row of a sheet contains the profile for a single object. Profiles are linearly-interpolated such that each measured position along a profile is 1px from the previous.

Note: Objects must either form a single line (i.e. not contain multiple branches) or reduce to a single line during skeletonisation. No profile will be recorded for any objects which fail this requirement.

Parameters

Input objects: Objects for which intensity profiles will be generated.

Measure another image: Include another image from the workspace to be measured. Each separate image will be measured at the same spatial points and be saved to a separate sheet of the .xlsx file.

Input image: Image for which the intensity profile will be measured. Results from this profile will be added to a separate sheet in the .xlsx file.

Include timepoints: Include a column recording the timepoint that the objects were present in.

Save name mode: Controls how saved profile file names will be generated.

File name: Filename for saved distance file. Note: Care should be taken with this when working in batch mode as it's easy to continuously write over output files from other runs.

Append series mode: Controls if any series information should be appended to the end of the filename. This is useful when working with multi-series files, as it should help prevent writing files from multiple runs with the same filename. Series numbers are prepended by "S". Choices are: None, Series name, Series number.

Append date/time mode: Controls under what conditions the time and date will be appended on to the end of the profile file filename. This can be used to prevent accidental over-writing of files from previous runs:

Add filename suffix: A custom suffix to be added to each filename.