FastQCFastQC Report
Wed 2 Dec 2015
MT.SE.preprocessed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.SE.preprocessed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951440
Sequences flagged as poor quality0
Sequence length40-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACAAAG305011.5629996310416923No Hit
GGATATGTAAAGGCAACTTAAATGAATACCATGACAATAATGATAATTCA150530.7713790841634895No Hit
TAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACAAA120960.6198499569548641No Hit
TTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACAA91450.4686282950026647No Hit
AAGCTGGTGTGATGCATTATAATTTATTAAGATATGAAGAAGACAAAGAG64200.3289878243758455No Hit
CAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACAAA59630.3055692206780634No Hit
ATGCAACCACTCGTGATTCATCAAAATATCGAACGCAACTTGCAGTGTAT52070.2668285983683844No Hit
TGTAAGCTGGTGTGATGCATTATAATTTATTAAGATATGAAGAAGACAAA51320.26298528266305904No Hit
GGTGTGATGCATTATAATTTATTAAGATATGAAGAAGACAAAGAGTAATC49820.25529865125240847No Hit
ATAACATTTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAA49310.25268519657278726No Hit
AACATTTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGT48210.24704833353831018No Hit
CTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACAA48030.24612593776903208No Hit
GTAAAGGCAACTTAAATGAATACCATGACAATAATGATAATTCAAAACAG44900.2300865002254745No Hit
AGTAACGGCGAGTGAAAAGGATAAAGCGCAAAATTAAAATCTCAACTGAA35220.180482105522076No Hit
GTGATAAGTATAATGATTCGATGGACATTGTATTTTTGAATATGTTTTTC34160.17505021932521625No Hit
AGCAATACAGTGATTGGTTTTTCGATCAAATACTATGGAATTTAATTTGT33970.17407657934653384No Hit
AGTATGATATGCAACCACTCGTGATTCATCAAAATATCGAACGCAACTTG31990.16393022588447506No Hit
ATAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACAA31900.16346902799983604No Hit
TAGTATGATATGCAACCACTCGTGATTCATCAAAATATCGAACGCAACTT28720.14717336940925674No Hit
CAAGGGTCAATAAGGAAAAATAACATTGTCATTTTTTCTGATGTTTGAAA28690.14701963678104374No Hit
GATAAGTATAATGATTCGATGGACATTGTATTTTTGAATATGTTTTTCTA28350.14527733366129628No Hit
GAAAGTGTATAGACATATAAACGGCAGTAATAATAATAAAATATTATATT28150.14425244947320953No Hit
AGACATATAAACGGCAGTAATAATAATAAAATATTATATTATTACTTGTA27060.13866683064813676No Hit
TAGCAGTTGTTAGATAATATGGGTGTTTTATTGATTAGTATGTACTATTT25120.12872545402369534No Hit
GATATGCAACCACTCGTGATTCATCAAAATATCGAACGCAACTTGCAGTG24130.12365227729266592No Hit
AGCAGTTGTTAGATAATATGGGTGTTTTATTGATTAGTATGTACTATTTT23830.12211495101053581No Hit
AGGAGTATAACATTTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGA21670.11104620177919894No Hit
AAGGAGTATAACATTTAGGCGAGGATATAAGTGATTAAACTTGAATCCGG20540.10525560611650883No Hit
GTGATGCATTATAATTTATTAAGATATGAAGAAGACAAAGAGTAATCTTG20510.10510187348829582No Hit
TTTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACA19950.10223219776165293No Hit
CCTCAAGGGTCAATAAGGAAAAATAACATTGTCATTTTTTCTGATGTTTG19880.10187348829582257No Hit
CTTAGGCGAGGATATAAGTGATTAAACTTGAATCCGGAATGAAAGTAACA19680.10084860410773581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT2957.2759576E-1296.3879394-95
CTAATCT3707.965355E-963.51977590-91
TTGGTCC4454.2018655E-1061.5800192-93
TCTCCCT4856.493792E-1058.62771294-95
CTCCCTA2852.0936568E-951.40416794-95
CGTATGC7600.049.9730392-93
TATGCCG8150.049.4331594-95
CTCCTTG5751.6007107E-1049.17831888-89
GTATGCC8200.044.21117892-93
TCGTATG8800.042.16058390-91
AATCTCC6651.4735633E-841.2076892-93
ATGCCGT8550.040.69496594-95
CCTTGGT6701.8788342E-840.0892490-91
GCCGTCT3802.6609996E-838.55312394-95
GATATGT30400.038.1213042
TATGTAA31100.038.112924
TATACTC3801.5643309E-1037.48065684-85
ATATGTA31150.037.324643
TGTAAAG32000.036.9230276
GGGAACG4851.3024971E-436.64232394-95
Produced by FastQC (version 0.11.3)