FastQCFastQC Report
Wed 2 Dec 2015
MT.R2.preprocessed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.R2.preprocessed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9119940
Sequences flagged as poor quality0
Sequence length40-101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG1108491.2154575578348104No Hit
TGCCAATAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT285440.31298451524900384No Hit
TGCCAGTAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT272910.29924538977230114No Hit
CCTGTTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG259980.28506766491884816No Hit
CCCTATCGATTTTAAACAGAAAAGAGAACTCTGTTTTGAATTATCATTAT259480.2845194156979103No Hit
ACCATTCAATCTTTGAGGATCGATAAACCAATAGTTATGTTTGAAAATAG231150.2534556148395713No Hit
TGCCATTAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT210060.23033046270041252No Hit
CCTATCAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG150440.16495722559578244No Hit
TGCCGATAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT142630.15639357276473312No Hit
CCCTACCGATTTTAAACAGAAAAGAGAACTCTGTTTTGAATTATCATTAT141870.15556023394890756No Hit
CCTACTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG129440.14193075831639243No Hit
CCCTGTCGATTTTAAACAGAAAAGAGAACTCTGTTTTGAATTATCATTAT123350.13525308280536932No Hit
CCTTTTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG118210.12961708081412818No Hit
ACCACCAAGATCTGTACTAATGCATGATCGAAACAAGATTACTCTTTGTC109630.12020912418283453No Hit
CTCACCATGTATAGGAATATTAACCTATTTCCTTTTCAAACATCAGAAAA109370.11992403458794686No Hit
ATTAGGACATGAATTTCATAATTGAATTGTTTTTAAAGGGCATTCTAAAG108700.11918938063189012No Hit
TCCTATTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATA108210.11865209639537103No Hit
TGCCACTAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT103190.11314767421715495No Hit
TCTTCATATCTTAATAAATTATAATGCATCACACCAGCTTACAAGTAATA102900.11282968966901097No Hit
GTACTAATGCATGATCGAAACAAGATTACTCTTTGTCTTCTTCATATCTT102330.11220468555714182No Hit
CACCATGTATAGGAATATTAACCTATTTCCTTTTCAAACATCAGAAAAAA95440.10464981129261815No Hit
CCCTATTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATA94900.10405770213400528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA190600.063.6634294-95
TCTCCCT235550.060.0024694-95
CTAATCT300950.054.75192690-91
CCTATTA189150.054.5272871
CCTACCG30750.054.0457082
AATCTCC308600.053.7156192-93
GTCCGTG155850.051.97390494-95
AACTAAT322700.049.8675688-89
GGTCCGT167150.049.52066494-95
CCCTACC32550.049.5175971
TAAATCA363000.049.3715026
ATCTCCC242500.049.06331692-93
GCTACAA307100.049.0274280-81
ATAACTA324500.048.81876486-87
TTAAATC331850.048.5449075
AATCAAA409550.047.5832528
TAATCTC309100.047.4730890-91
CGATTTT238000.047.3180967
GTAAACT82850.047.174426
ATCAAAT414400.046.955829
Produced by FastQC (version 0.11.3)