FastQCFastQC Report
Wed 2 Dec 2015
MT.R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16493393
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG1235690.7492030293584832No Hit
CACTAGTCAATTTCACATTCTAATATTTAACTCTCTTTTCAAAGTTCTAT668860.405532081846349No Hit
TGCCAATAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT556670.33751090512425186No Hit
TGCCAGTAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT527670.3199281069698636No Hit
CCCTATCGATTTTAAACAGAAAAGAGAACTCTGTTTTGAATTATCATTAT441000.26737979262362815No Hit
TGTACGTACGTGTTACTTATACTTAGATTTGCATGGCTTAGTCCTTTAAA378310.22937063344091782No Hit
TGCCATTAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT371060.22497493390232076No Hit
CTAGTTTTACTACAGTTATCCACATATAGGTTTGAAACTGTTTTAAGTTT369310.22391390297921113No Hit
CGCCCCTATACTCATATTTGACAAACGATTTGCACGTCAGTATTGCTACG357810.21694141405591924No Hit
GTCCTGGTGTCTTAATACAACAACACTTTTTATGGTATCTAATAATGAGT308070.18678388370422025No Hit
CCTGTTAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG288640.1750034089407801No Hit
AGTCAATTTCACATTCTAATATTTAACTCTCTTTTCAAAGTTCTATTTCA280150.1698558932052368No Hit
GCCCCTATACTCATATTTGACAAACGATTTGCACGTCAGTATTGCTACGA278220.16868572767289303No Hit
TGCCGATAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT274770.16659398099590544No Hit
ACCATTCAATCTTTGAGGATCGATAAACCAATAGTTATGTTTGAAAATAG272670.1653207438881739No Hit
CTTTTTCGGTTCTCACTAGTCAATTTCACATTCTAATATTTAACTCTCTT265990.16127063727881824No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC257950.15639595806636028Illumina Single End PCR Primer 1 (100% over 50bp)
ACCACCAAGATCTGTACTAATGCATGATCGAAACAAGATTACTCTTTGTC243510.1476409371922442No Hit
CCCTACCGATTTTAAACAGAAAAGAGAACTCTGTTTTGAATTATCATTAT232890.14120199524743027No Hit
GTACTAATGCATGATCGAAACAAGATTACTCTTTGTCTTCTTCATATCTT217280.13173759941329233No Hit
TCCCGCATCACATATTCTGCTTACCAAAAAATGTCCACAAATAGAAAAAC214900.13029459735786325No Hit
GGTGTCTTAATACAACAACACTTTTTATGGTATCTAATAATGAGTGTATA214150.12983986981938767No Hit
CCCTGTCGATTTTAAACAGAAAAGAGAACTCTGTTTTGAATTATCATTAT210480.1276147363977806No Hit
TGCCACTAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT195790.11870813967750603No Hit
TCCATTAATAGTTGTATCTATGAAGATACACTGCAAGTTGCGTTCGATAT192210.11653757356051603No Hit
CCCGCATCACATATTCTGCTTACCAAAAAATGTCCACAAATAGAAAAACA186800.11325747224964566No Hit
GCCGTTTATATGTCTATACACTTTCAGCGCCATTAATTTTCAAGGCTATT174640.10588482309249528No Hit
TGCCTATAAACTTTATTTTATTATGAGCTTTGTTACTTTCATTCCGGATT171530.10399921956628329No Hit
TCTTCATATCTTAATAAATTATAATGCATCACACCAGCTTACAAGTAATA169550.10279873886470782No Hit
CTCACTAGTCAATTTCACATTCTAATATTTAACTCTCTTTTCAAAGTTCT169160.10256228054470054No Hit
CCTATCAAATCAAATAAATACTACTTCTCTAAACAAATTAAATTCCATAG167190.10136786287697142No Hit
GTTCCTTAATTGTTAGGCATCTCTTTTTCGGTTCTCACTAGTCAATTTCA166230.1007858116277227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACT135850.056.763536
TAAACTT526150.056.0591937
TAAATCA410900.054.060276
AACTTTA547000.053.90169
CCTATTA237600.053.274021
TTAAATC374550.052.975015
CCTACCG47700.052.532342
AGTAAAC122600.051.7924355
AAACTTT574100.051.5150268
CGATTTT314350.051.5039987
GCCAGTA118200.050.7104072
GGTGTGC181750.050.156666
TGTGCTC250150.050.0188758
GTGTGCT257550.048.9894077
GTGCTCT257200.048.351939
AATCAAA507300.047.7947658
AAATCAA504350.047.6532867
TGCCAGT120300.047.5795751
CTACCGA53850.046.8850633
CCTATCG98950.046.8586772
Produced by FastQC (version 0.11.3)