FastQCFastQC Report
Wed 2 Dec 2015
MG.SE.preprocessed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG.SE.preprocessed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199766
Sequences flagged as poor quality0
Sequence length40-103
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA950.001524875576.9616992-93
TTAGAAT1253.2211177E-452.70279396-97
TCGGAAG4400.029.94823
GTATTGG2150.00875707629.01009696-97
ATCGGAA4800.028.106152
AACTTAT1855.9385588E-527.7454584-85
CTGCTTT4107.0623076E-427.46279190-91
AGTGTAT1250.00804405827.3755184-85
TTACTAC1550.00968840126.349988-89
AGAAATA2701.0563142E-425.49059790-91
AGAGCGT4000.023.530738
AACACTT2700.0019118823.15819492-93
GATCGGA6400.022.0600591
ACATTTA1650.002599461721.96930380-81
GAGGGTA2350.021.3612326-27
CGGAAGA6200.021.2535634
AGGGTAG2300.019.77945528-29
AAGAGCG5800.019.4737077
GTAGGGA2150.018.9705118-19
GGAAGAG6900.018.6427065
Produced by FastQC (version 0.11.3)