FastQCFastQC Report
Wed 2 Dec 2015
MG.R2.preprocessed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG.R2.preprocessed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13108258
Sequences flagged as poor quality0
Sequence length40-103
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCCC90250.07.97524556
AACCCCA94150.07.72889387
ACTAGTG8802.5706153E-47.531358796-97
ACCCCAA96500.07.45871358
CCCCAAA97650.07.20887661
AAAACCC111900.06.4675665
CCCAAAA114100.06.3428622
CCAAAAC131000.05.7660973
CAAAACC148650.05.0282494
Produced by FastQC (version 0.11.3)