FastQCFastQC Report
Wed 2 Dec 2015
MG.R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG.R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14699402
Sequences flagged as poor quality0
Sequence length103
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG473360.322026705576186Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG293690.19979724345248875Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG243850.073.3025053
AGAGCGT208700.072.1029368
GATCGGA258500.069.7867661
ATCGGAA259350.069.145972
CGGAAGA267600.065.800524
AAGAGCG252500.064.5316857
GAGCGTC241700.062.11689
GAAGAGC275000.059.5680276
GGAAGAG335750.052.4157265
TCGGGAG98850.043.6125343
GGGAGAG100750.041.9722375
TAGGGAA156150.039.98183418-19
TGTAGGG176550.039.61736716-17
GTAGGGA154750.039.3721618-19
CGTGTAG178300.039.20232814-15
CGGGAGA101300.038.776164
TAGATCT173400.038.5781932-33
TCGTGTA168100.038.0330414-15
AGATCTC247800.037.9442332-33
GTGTAGG182050.037.8078716-17
Produced by FastQC (version 0.11.3)