FastQCFastQC Report
Mon 5 Oct 2015
MT.SE.trimmed.rna_filtered.hg38.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.SE.trimmed.rna_filtered.hg38.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541262
Sequences flagged as poor quality0
Sequence length40-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTCACGTGTACGTAGTACGTACGCATACACGCATGTACGTATATAGT15670.28950859288108166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG1250.065.6316490-91
CGCTAGT1300.063.1073490-91
GCTAGTG1350.061.7406492-93
CTAGTGC1400.059.53561892-93
CACGTGT1950.052.8440256
ACGTCTA1700.047.00270576-77
GACGCTA1800.046.71894588-89
CGTGTAC2300.044.802548
GTGTACG2350.043.8492979
TCACGTG2550.041.9068075
TAGTGCG2050.041.66722594-95
ACGTCAC2400.041.3455542
ACGTGTA2550.040.4101337
GTCACGT2700.038.1651274
TACGTCT2100.038.0498176-77
CTCAGAC2250.036.1450184-85
AGTGCGT2350.034.83350894-95
CTCGCGT2100.034.73203352-53
CACGTCA3050.033.7855221
CGCGTAG2150.033.25580654-55
Produced by FastQC (version 0.11.3)