FastQCFastQC Report
Mon 5 Oct 2015
MT.R2.trimmed.rna_filtered.hg38.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.R2.trimmed.rna_filtered.hg38.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9711247
Sequences flagged as poor quality0
Sequence length40-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATC6900.017.0762182
ATATAGT45250.016.7249686
TATAGTA43200.015.6303947
ACCTAGT3751.04204446E-714.5016496
TAGATCG11050.012.3034353
CCTAGTT3752.3990402E-512.0847077
ACTAACC36800.012.07688162-63
ACTAGTG11250.012.04362572-73
GTACTAG12050.011.59977570-71
TCTAGGC7600.011.08253594-95
GGGCCTC21600.010.77950638-39
ATAGTAA68250.010.7567188
TGGGAGT44100.010.5843959
CGGACGG50050.010.4126481
ACGTTAC34900.010.3880013
CCTAAAG39700.010.3876741
TTGACAG69200.010.2816067
TCCTAGT7555.178663E-910.2039759
CTAGTCG4902.3575652E-510.173352
CGGGGGT17600.09.91323710-11
Produced by FastQC (version 0.11.3)