FastQCFastQC Report
Mon 5 Oct 2015
MT.R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10691656
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGT51550.015.5733136
CCTAGTT4702.6011548E-1015.1607417
CTAGATC8500.015.0924332
TATAGTA46900.014.686637
CGCCTAA13450.014.4835141
ACCTAGT5258.913048E-1114.4771686
TCTACCT34200.012.500967
TGATCTA47600.011.4784414
TCTAGGC11200.011.24680894-95
ACTAACC40800.011.17252162-63
CTAGGCC10550.011.03862894-95
CCTAAAG42700.011.0158931
ATCTACC41400.011.0152376
CTCTAAT23300.011.0115941
GATCTAC43950.010.8100355
AACCTAG14450.010.5212695
ACTCTAG12200.010.5197592-93
GATCGAT73050.010.4710835
TTGACAG79200.010.2564717
ACGTTAC35200.09.9886673
Produced by FastQC (version 0.11.3)