FastQCFastQC Report
Mon 5 Oct 2015
MT.R1.trimmed.rna_filtered.hg38.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.R1.trimmed.rna_filtered.hg38.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9711247
Sequences flagged as poor quality0
Sequence length40-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAAT39250.025.9815863
CTGGTTA42800.025.6511291
TGGTTAA43150.022.462292
GTTAATA50500.021.1941934
TGACTCG30350.017.1029938
TTGACTC37150.015.208937
CCTAGCG22700.014.79256336-37
TAAGGTG18400.014.4797999
GCTAGAT10200.014.4112675
CCCGGGT17900.014.3710131
TTAATAC73100.014.1389625
TAGACTC40200.013.7122139
CAGCTAG7850.013.4589313
TAATACA76900.013.3208176
GACTCGC39900.013.1245479
AATAGAC41700.012.9986537
GGTTGAC49500.012.6207765
ACTAGGG2554.703969E-412.6098598
GCCCTAG26650.012.49969334-35
CTTAGGG15150.012.4315011
Produced by FastQC (version 0.11.3)